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MICROBIAL METABOLISM

MICROBIAL METABOLISM. RINGKASAN PROSES METABOLISME. SUBSTRAT (Donor Elektron). PRODUK TEROKSIDASI. KATABOLISME (Peruraian). SINTESIS ATP. ATP (Pembawa Energi). KONSUMSI ATP. ANABOLISME (Biosintesis). MOLEKUL SEDERHANA. BAHAN SEL. Red. ATP. R. Oxidation process (Exergonic).

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MICROBIAL METABOLISM

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  1. MICROBIAL METABOLISM

  2. RINGKASAN PROSES METABOLISME SUBSTRAT (Donor Elektron) PRODUK TEROKSIDASI KATABOLISME (Peruraian) SINTESIS ATP ATP (Pembawa Energi) KONSUMSI ATP ANABOLISME (Biosintesis) MOLEKUL SEDERHANA BAHAN SEL

  3. Red ATP R Oxidation process (Exergonic) Essential endergonic task Endergonic process Exergonic process Endergonic process Exergonic process Ox + e- ADP + Pi P MICROBIAL METABOLISM

  4. FOUR BASIC NEEDS OF METABOLISM PHOTOTROPHS Light ENERGY ATP CHEMOTROPHS Reduced inorganic compounds LITHOTROPHS AEROBES O2 Stored electrons (e.g., NADH) ELECTRON ACCEPTOR ELECTRON DONOR Reduced organic compounds Alternate e- acceptors ANAEROBES ORGANOTROPHS HETEROTROPHS CO2, HCO3- CARBON AUTOTROPHS Synthesized cellular components Reduced carbon intermediates

  5. METABOLIC CLASSIFICATION

  6. HIGH-ENERGY PHOSPHATE BONDS • ATP—ENERGY CURRENCY OF CELL

  7. FERMENTATION Organic compound Fermentation products RESPIRATION Carbon flow Carbon flow Carbon flow Electron flow Organic compound CO2 ANAEROBIC RESPIRATION Internal Oxidation- Reduction Electron flow Organic compound CO2 O2 Electron flow NO3-, SO42-, CO32-, etc. THREE MAIN MODES OF METABOLISM

  8. (CH2O)n NADP+ H2O [ADP + Pi] Cahaya CH2OP Khlorofil CO2 + H20 NADPH O2 ATP Reaksi Gelap Reaksi Terang Kemosintesis (Bakteri Hidrogen) : H2+½ O2 [ADP + Pi] (CH2O)n NADP+ CH2OP ATP CO2 + H20 NADPH H2O Reaksi Gelap Reaksi Gelap

  9. H2O [ADP + Pi] (CH2O)n NADP+ Cahaya Khlorofil CH2OP CO2 + H20 O2 ATP NADPH Reaksi Gelap ReaksiTerang Kemosintesis (Bakteri Hidrogen) : H2+½ O2 [ADP + Pi] (CH2O)n NADP+ CH2OP ATP CO2 + H20 NADPH H2O Reaksi Gelap Reaksi Gelap

  10. BIOSYNTHESIS & NUTRITION Anabolism & Catabolism

  11. sum = metabolism Metabolism =Catabolism + Anabolism Pay particular attention to those words in bold in this figure.

  12. More generally, sun or reduced inorganic chemicals (ocean vents). More generally, photoautotrophs (or just autotrophs). E.g., glucose More generally, chemoheterotrophs. Note that bacteria don’t have mitochondria: aerobic bacteria “are” mitochondria!

  13. Ezyme with Cofactor

  14. ATP – Energy Currency of Cells

  15. ATP, ADP, and Metabolism

  16. Oxidation-Reduction (Redox)

  17. Oxidation-Reduction (Redox)

  18. Oxidation-Reduction (Redox) Note that 2 hydrogen ions are removed along with the 2 electrons (only one H+ ends up attached to NAD+, hence the charge change). Note NAD+ (not “NAD”).

  19. Overview of Metabolism

  20. Glucose NAD+ NADH ATP ATP Pyruvate Glycolysis (see Fig. 6.14) An Enzyme and Coenzyme Mediated Catabolic Pathway

  21. Glycolysis (see Fig. 6.14) An Enzyme and Coenzyme Mediated Catabolic Pathway

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