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Avena genetic resources for quality in human consumption (AVEQ)

Avena genetic resources for quality in human consumption (AVEQ). Partner 1 2 IHAR -PIB Laboratory of Quality Evaluation of Plant Materials Institute of Plant Breeding and Acclimatization - National Research Institute , Radzików, PL 05 - 870, Poland.

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Avena genetic resources for quality in human consumption (AVEQ)

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  1. Avena genetic resources for quality in human consumption (AVEQ) Partner 12 IHAR -PIB Laboratory of Quality Evaluation of Plant Materials Institute of Plant Breeding and Acclimatization - National Research Institute, Radzików, PL 05-870, Poland AVEQ Final Meeting – BucharestOctober 19-21, 2010

  2. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre The objective: Characterise carbohydrates - starch and dietary fibre in genetic resources of Avena. AVEQ Final Meeting – Bucharest October 19-21, 2010

  3. WP 7. Task 1, Sub-task 4 . Analysis of starch - selected method -amylase/amyloglucosidase method: ICC Standard no. 128/1and168 or AACC 76-13 or AOAC 996.11 AVEQ Final Meeting – Bucharest October 19-21, 2010

  4. WP 7. Task 1, Sub-task 4 . Analysis of dietary fibre - selected method Enzymatic-Chemical Uppsala Method (AOAC 994.13 or AACC 32-25) Gas chromatographic-colorimetric-gravimetric procedure • TDF = neutral sugar residues + uronic acids + Klason lignin • Principle • 1. Measurement of NSP as neutral sugar residues by GLC • (Englyst and Cummings procedure, 1988) • starch removal by specific enzymes • precipitation of soluble polymers by ethanol • hydrolysis of polysaccharides to monosaccharides with H2SO4 • reduction and derivatisation • 2. Measurement of uronic acid by colorimetry (Scottmethod, 1979) • 3. Measurement of Klason lignin by gravimetric method as the sulphuric acid resistant residue (Theander et al.,1995)

  5. Dietary Fibre Constituents I. Non-starch polysaccharides & oligosaccharides Cellulose Non-cellulosic polysaccharides • Polyfructoses • inulin • oligofructans • Galactooligosugars • Hemicelluloses • arabinoxylans • arabinogalactans • β-glucans • Pectins • Gums • Mucilages IV. Substances associated with NSP & lignin complex in plants II. Analogous Carbohydrates Waxes Phytates Cutin Suberin Saponins Tannins Resistant starch Synthesized carbohydr. Indigestible dextrins III. Lignins AVEQ Final Meeting – Bucharest October 19-21, 2010

  6. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre Material analysed from the year 2008 - 60 samples of selected accessions from trial in Bulgaria - 61 samples of selected accessions from trial in Estonia - 2 samples of wild accessions from trial in Estonia - 6 samples of wild accessions from trial in Poland - 53 samples of standards from trial in Estonia Total no of accessions: 182 AVEQ Final Meeting – Bucharest October 19-21, 2010

  7. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre I set - selected 60 accessions from Bulgaria trial Range 51.9 – 64.1%; avg – 56.3%; CV – 4.3%

  8. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre I set - selected 60 accessions from Bulgaria trial TDF - range 9.4–18.2% avg - 12.0%; CV - 10% 5.0 2.6 6.1 14.1 NSP - range 6.1–14.1% avg - 8.8 %; CV - 13% Uronic acids - range 0.14–0.32% avg – 0.23%; CV - 18% Lignin - range 2.6–5.0% avg - 3.3%; CV - 13%

  9. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial Range 48.9 – 64.9%; avg – 58.7%; CV – 5.9%

  10. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial TDF - range 9.0–15.8% avg - 11.2%; CV - 12% 4.4 1.8 11.8 6.5 NSP - range 6.5–11.8% avg - 8.2 %; CV - 13% Uronic acids - range 0.12–0.24% avg – 0.17%; CV - 16% Lignin - range 1.8–4.4% avg - 2.8%; CV - 22%

  11. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre II set - selected 61 accessions from Estonia trial plus 2 wild Avena samples TDF - range 9.0–15.8% avg - 11.2%; CV - 12% NSP - range 6.5–11.8% avg - 8.2 %; CV - 13% Uronic acids - range 0.12–0.24% avg – 0.17%; CV - 16% Lignin - range 1.8–4.4% avg - 2.8%; CV - 22%

  12. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre Polish wild species AVEQ Final Meeting – Bucharest October 19-21, 2010

  13. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre - standards

  14. WP 7. Task 1, Sub-task 4 . Relationship between NSP on TDF content TDF [% DM] NSP [% DM] AVEQ Final Meeting – Bucharest October 19-21, 2010

  15. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre • Plans for the next four months: • Additional standard samples (53 samples of 11 cultivars from field experiment in Estonia) at present are analyzing for dietaryfibreand they shouldbe completed within 10 day from now. • Analyses for starch and TDF (NSP only) in 70 selected accessions from trial inPoland (2008) and France, Sweden and Italy (2009). AVEQ Final Meeting – Bucharest October 19-21, 2010

  16. WP 7. Task 1, Sub-task 4 . Analyses for starch and dietary fibre • This work has been carried out by : • Anna Fraś • Olga Jablonka • Danuta Boros • With appreciable help of two technicians: • Magdalena Bodzon • Monika Skonieczna AVEQ Final Meeting – Bucharest October 19-21, 2010

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