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Diffusion & Osmosis

Diffusion & Osmosis. Solvent = solute(s) + solvent 1. examine diffusion of solutes Ex 6.3-dialysis

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Diffusion & Osmosis

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  1. Diffusion & Osmosis • Solvent = solute(s) + solvent • 1. examine diffusion of solutes • Ex 6.3-dialysis • 􀁡2. examine diffusion of the solvent(osmosis) • Eg.6.2

  2. Diffusion exercise • Diffusion exercise • 􀁡Movement of molecules from high concentration to low concentration(down the concentration gradient) • 􀁡diffusion through solid (agar plates) liquid and gas

  3. Factors influencing diffusion • 􀁡Rate and temperature (increase diffusion w/ increase temp) • Molecular weight (increase diffusion w/ decrease MW) • solute MW Potassium permanganate = 294.21 and Janus Green = 511.09 • Ex 6.1- Try different temperatures

  4. Temperature and diffusion • Ex 6.1- Prepare the 2 plates of AGAR • Add a drop of each dye into the well. • Place at different temperatures • Measure the diffusion diameter of the well at different time intervals

  5. Osmosis experiment • Ex 6.2 • Prepare bags as instructed in the book. • Place them at the respective beakers. • Check initial wt and wt periodically to see if osmosis has occured

  6. Diffusion permeability of Membranes, • 10 ml of solution (starch + protein + NaCl + Na2SO4) • test water after 1 hr for: • Starch w/ Iodine (blue-black) • Cl - w/ AgNO3 (milky white ppt.) • SO4 • - w/ BaCl2 (white ppt.) • Protein w/ Biuret (violet)

  7. Tonicity exercise • plant cells (Elodea leaf cells) • Turgid􀁡hypotonic solution,􀁡water enters cell􀁡chloroplasts are at the periphery of thecell

  8. Tonicity-Ex 6.4 B Plasmolyzed 􀁡hypertonic solution 􀁡water leaves the cell 􀁡chloroplasts are at the center of the cell

  9. TONICITY-EX 6.4 A • animal cells (erythrocytes) • 􀁡normal (0.85% NaCl) isotonic, no net movement of water • 􀁡crenated (10% NaCl)hypertonic,water leaves • 􀁡hemolyzed (0.45% NaCl),hypotonicwater enters

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