1 / 12

Rebecca M. Hamner Advisor: Wilson Freshwater

The use of DNA markers to identify and explore the genetic diversity of lionfishes Pterois miles and Pterois volitans. Rebecca M. Hamner Advisor: Wilson Freshwater. P. miles P. volitans Mixed Invasive Pterois sp. Current Data

len
Télécharger la présentation

Rebecca M. Hamner Advisor: Wilson Freshwater

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. The use of DNA markers to identify and explore the genetic diversity of lionfishes Pterois miles and Pterois volitans Rebecca M. Hamner Advisor: Wilson Freshwater

  2. P. miles P. volitans Mixed Invasive Pterois sp.

  3. Current Data • Disagreement between mitochondrial DNA and meristic identities • 14 nuclear DNA loci lack species specific variation for P. miles and P. volitans Act-2 AldB Cam-3 CK CKA7 ChymB-6 ITS LDH LDHA6 Mlc-3 Ops-1 RP1 RP2 SSU

  4. Objective I • Investigate hybridization using Randomly Amplified Polymorphic DNAs (RAPDs) to develop a nuclear DNA marker to distinguish between Pterois miles and Pterois volitans

  5. MethodsRandomly Amplified Polymorphic DNAs (RAPDs) 1. Polymerase Chain Reaction 5’ 3’ 4 1 2 3 Template DNA Primer sites Copied DNA fragments

  6. 2. Gel Electrophoresis A Size marker ? B longest fragments shortest fragments

  7. Objective II • Examine the genetic diversity of North Carolina lionfish to determine their source population ?

  8. ? Methods NC lionfish PCR & Sequence Reaction ABI 3100 Genetic Analyzer Phylogenetic tree or Cluster analysis

  9. Questions? Leo (NC lionfish) at CMS

  10. Mer = m Mito = v Nuc = v Mer = v Mito = m Nuc = m Mer = m Mito = v Nuc = m Mer = v Mito = m Nuc = v Meristic and DNA disagreement Meristic characters not fixed for P. miles and P. volitans Nuclear and mitochondrial DNA disagreement -Hybridization -Incomplete lineage sorting Mer = meristic counts Mito = mitochondrial DNA Nuc = nuclear DNA

  11. 2. Gel Electrophoresis Partis, L. and R. J. Wells. 1996. Identification of fish species using random amplified polymorphic DNA (RAPD). Molecular and Cellular Probes, 10: 435-441.

More Related