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How enzyme function

How enzyme function. Chemical reaction. The cells carries out many chemical reactions. A + B C +D reactants products All the reactions that occur in a cell have an enzyme. Enzymatic action.

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How enzyme function

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  1. How enzyme function

  2. Chemical reaction • The cells carries out many chemical reactions. A + B C +D reactants products • All the reactions that occur in a cell have an enzyme.

  3. Enzymatic action • Enzymes are organic catalysts that speed metabolic reactions. • Most of the enzymes are proteins. • The reactants in an enzymatic chemical reaction are called substrates. E + S ES E + P

  4. Enzymes have specific shape accommodating the shapes of their substrate. • Enzymes can be used over and over again. Active site

  5. Catalase • Catalase speeds the breakdown of hydrogen peroxide to water and oxygen 2H2O2 2H2O + O2 catalase

  6. Factors affecting enzyme activity • Temperature • Concentration (of enzyme or substrate) • pH

  7. Temperature • Cold (ice bath)— slow chemical reaction • Warm— speed chemical reaction • Hot (boiling water bath)— enzyme denature

  8. Concentration (of enzyme or substrate) • Low—slower chemical reaction • High— faster chemical reaction

  9. pH • pH<7 acidic • pH=7 neutral • pH>7 alkline • Each enzyme— optimum pH speed the reaction best • Low / high—affects hydrogen bonding and the structure of enzyme, reduced activity

  10. Summary

  11. Experiments • 1. Catalase Activity: what does the height of the bubble column mean? what’s the function of catalase? page 59 • 2. Effect of Temperature: finish table 5.2 page 60 • 3. Effect of Enzyme Concentration: finish table 5.3 page 61 • 4. Effect of pH on Enzyme Activity: finish table 5.4 page 62

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