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Mukund Thattai NCBS Bangalore

genetic networks in theory and practice. Mukund Thattai NCBS Bangalore. Ecoli: The best understood living organism. 1 M. 1 M. Ecoli: Complex matter that can be engineered. Network biology. protein. mRNA. DNA. RNAP. gene. Network biology. protein. mRNA. regulator. DNA. RNAP.

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Mukund Thattai NCBS Bangalore

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  1. genetic networks in theory and practice Mukund Thattai NCBS Bangalore

  2. Ecoli: The best understood living organism 1M

  3. 1M Ecoli: Complex matter that can be engineered

  4. Network biology protein mRNA DNA RNAP gene

  5. Network biology protein mRNA regulator DNA RNAP gene

  6. A biological wish-list Embryonic development requires complex regulation [] Amplification [ ] Noise reduction [ ] Memory [ ] Oscillations  Alberts et al., 1994

  7. Regulated genes Activation Inhibition (D) X [] Amplification [ ] Noise reduction [ ] Memory [ ] Oscillations

  8. Negative feedback [] Amplification [] Noise reduction [ ] Memory [ ] Oscillations

  9. Positive feedback [] Amplification [ ] Noise reduction [] Memory [ ] Oscillations

  10. Flip flop [] Amplification [ ] Noise reduction [] Memory [ ] Oscillations

  11. Hysteretic oscillator [] Amplification [ ] Noise reduction [ ] Memory [] Oscillations

  12. Ring oscillator [] Amplification [ ] Noise reduction [ ] Memory [] Oscillations

  13. Emergent network properties Amplification Noise reduction Memory Oscillations These predictions have been verified experimentally in engineered genetic networks

  14. Engineering Synthetic Networks Inducers: Off-the-shelf chemicals Reporters: Fluorescent proteins Transcriptional regulators: DNA-binding proteins The host strain: Escherichia coli Promoters: DNA segments that control gene expression  parts.mit.edu

  15. Engineering Synthetic Networks The unity of biology allows us to combine modules in new and powerful ways  parts.mit.edu

  16. Negative feedback Noise reduction X PLtetO1 tetR*-egfp PLtetO1 tetR*-egfp  Becskei & Serrano, 2000  Figures adapted from Hasty et al, 2002

  17. Positive feedback Memory TMG Plac lacY Plac gfp  Ozbudak & Thattai et al., 2004

  18. Flip flop Memory IPTG PLs1con cIts gfp Ptrc-2 lacI Heat  Gardner et al., 2000  Figures adapted from Hasty et al, 2002

  19. Ring oscillator Oscillations PLtetO1 gfp PLtetO1 cI Plac lacI tetR PR  Elowitz & Leibler, 2000  Figures adapted from Hasty et al, 2002

  20. Getting your hands dirty...

  21. Elena Budrene New York Times

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