1 / 14

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Ma

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Materials. Carla Harkin. Dr Wolfram Brück Dr Catherine Lynch Dr Denis McCrudden. ChiBio: Work Package 2 Aims.

lyn
Télécharger la présentation

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Ma

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Materials Carla Harkin Dr Wolfram Brück Dr Catherine Lynch Dr Denis McCrudden

  2. ChiBio: Work Package 2 Aims • Development of efficient, economical and environmentally-friendly pre-treatment of crustacean shell wastes to extract chitinous material(Raw material sources: Europe/Asia/Africa). • Stabilisation of chitinous material for transfer to project partners (UMB, INS, TUM, FhG). • Development of assays for constant quality control.

  3. Pre-treatment overview Raw material Prep Optimisation of chemical chitin extraction methods Identification of novel microorganisms Optimisation of microbial chitin extraction methods QC Up-scaling

  4. Raw Material preparation

  5. Chemical Pre-treatment 7. QC Analysis

  6. Microbial Pre-treatment • Chemical extraction methods- costly and a hazard to the environment. • Biological extraction methods- more cost effective while posing less cause for environmental concern. • Microbial chitin extraction methods: • Direct use of enzymes (Brück et al, 2011; Jo et al, 2011). • Fermentation (Jung et al, 2005; Jung et al, 2007; Rao et al, 2000). Fermentation • Initial optimisation, adapting methods from literature. • Identification of novel microorganisms for fermentation.

  7. Fermentation Process Raw material + Inoculum Shaking Incubator QC Shaking Incubator Harvesting & drying Change of Inoculum (Jung et al., 2005; Jung et al., 2007a,b; Oh et al., 2007)

  8. Isolation of Novel Microorganisms Isolation from soil samples Screening for useful properties Small scale fermentation Identification Large scale fermentation Characterisation

  9. Initial Screening • Demineralisation–acid production. • Replicated fermentation method on micro scale. • Deproteinisation–protease production. • Casein agar plates. • Colorimetric protease assay to quantify activity (Vishwanatha et al, 2009). Fig 1: pH indication after addition of Methyl Red Indicator. L-r: negative yellow, negative orange, positive intense red. Fig 2: Produciton of zone of proteolysis in casein agar.

  10. Selection of Organisms

  11. To be done • Identification of isolates; PCR, sequencing- BLAST • Characterization of isolates: biochemical tests, staining etc. • Continue to meet chitinous material production targets. • Continuation of fermentation experiments using raw crustacean waste from Ireland, Indonesia and Tunisia.

  12. Future prospects • With select cultures, the aim is to scale-up fermentations to pilot scale to investigate the reality of industrial large scale fermentations in the breakdown of crustacean waste. • Development of an economic, environmentally friendly, waste reducing facility. • Profit-generating business opportunities for Irish Seafood Industry.

  13. References • Brück, W., M., Slater, J. W., Carney, B. F., 2011. Chitin and chitosan from marine organisms. Chitin, Chitosan, Oligosaccharides and their derivatives. Boca Raton: CRC Press. • Vishwanatha, T., Spoorthi, N., Reena, V., Divyashree B.C., Siddalingeshwara K.G., Karthic, J., Sudipta K.M., 2010. Screening of substrates for protease production from Bacillus lichenformis. International Journal of Engineering Science and Technology(2)11, pp. 6550-6554 • Jung, W.J., Jo, G. H., Kuk, J. H., Kim, K. Y., Park, R. D., 2005. Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serratia marcescens FS-3. Applied Microbiology and Biotechnology (71) pp. 234-237. • Jung, W.J., Jo, G. H., Kuk, J. H., Kim, Y. J., Oh, K. T., Park, R. D., 2007. Production of chitin from red crab shell waste by successive fermentation with Lactobacillusparacasei KCTC-3074 and Serratia marcescens FS-3. Carbohydrate Polymers (68) pp. 746-750. • Oh, K. T., Kim, Y. J., Nguyen, V. N., Jung, W.J., Park, R.D. 2007. Demineralization of crab shell waste by Pseudomonas aeruginosa F722. Process Biochemistry. (42) pp. 1069–1074. • Jo, G.H., Park, R.D., Jung, W.J., 2011. Enzymatic production of chitin from crustacean shell waste. Chitin, Chitosan, Oligosaccharides and their derivatives. Boca Raton: CRC Press. • Rao, M. S., Muñoz, J., Stevens, W. F., 2000. Critical factors in chitin production by fermentation of shrimp biowaste. Applied Microbiology and Biotechnology (54) pp. 808-813.

  14. Thank you very much for your attention.

More Related