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Isolation of Genomic DNA from Arabidopsis Leaves in Screening for T-DNA Knockout Plants

Isolation of Genomic DNA from Arabidopsis Leaves in Screening for T-DNA Knockout Plants. HC 70AL Spring 2009 April 9, 2009 Anhthu Bui. Arabidopsis Leaf. Collect in a microcentrifuge tube containing an Extraction buffer. Crush the leaf using a micropestle. Centrifuge. Cell Debris. Lysate.

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Isolation of Genomic DNA from Arabidopsis Leaves in Screening for T-DNA Knockout Plants

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  1. Isolation of Genomic DNA from Arabidopsis Leaves in Screening for T-DNA Knockout Plants HC 70ALSpring 2009April 9, 2009Anhthu Bui

  2. Arabidopsis Leaf Collect in a microcentrifuge tube containing an Extraction buffer Crush the leaf using a micropestle Centrifuge Cell Debris Lysate Precipitate Centrifuge Pellet (Nucleic acids, proteins, polysaccharides) Supernatant (Discarded) Dry Resuspend in TE buffer Nucleic Acids solution (genomic DNA, RNAs, proteins, polysaccharides) Why Don’t We Need a High Quality Genomic DNA for Screening T-DNA Knockout Plants?

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