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Autoinducers

Autoinducers. 3OC6HSL is AI-1 which interacts with LuxR to activate pLuxCl, which activates Luxpr. 3OC12HSL is PAI-1, which interacts with LasR to activate pLAS, which activates pLAS Furanosyl borate diester is AI-2, which interacts with LuxQ to activate pLux, which activates pLuxpr.

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Autoinducers

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  1. Autoinducers • 3OC6HSL is AI-1 which interacts with LuxR to activate pLuxCl, which activates Luxpr. • 3OC12HSL is PAI-1, which interacts with LasR to activate pLAS, which activates pLAS • Furanosyl borate diester is AI-2, which interacts with LuxQ to activate pLux, which activates pLuxpr.

  2. We used 4, 2-Hybrid systems (alpha gal4) • AB -- ZiF • CD – T25 and T18 • EF • VW Apparently there is a 3 hybrid system. So we could use 3, 2-hybrid systems and 1, 3-hybrid system.

  3. Gene of Interest A 000 tet TET Gene A Luxpr pLaxCl B 001 Lux R tet R B C Spo 1 xtR Gene B pLAS pLaS C 010 LAS R tet R A E Spo 1 rhyB Gene C pLUX pLux D 100 Lux Q tet R V D F xtR rhyB Gene D AB AB E 011 Gene E Spo1 CD CD F 101 Gene F xtR EF EF G 110 Gene G Rhy B VW AB H 111 W Hin Gene H

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