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biotin/streptavidin position on A tile of 2D DNA array (see next slide) staple:m13 5:1

All origami shown in this report is from the same annealing run that Massud showed in his 6/21/2011 weekly report. biotin/streptavidin position on A tile of 2D DNA array (see next slide) staple:m13 5:1 [m13]=10 nm SA:biotin staple 15:1

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biotin/streptavidin position on A tile of 2D DNA array (see next slide) staple:m13 5:1

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  1. All origami shown in this report is from the same annealing run that Massud showed in his 6/21/2011 weekly report

  2. biotin/streptavidin position on A tile of 2D DNA array (see next slide)staple:m13 5:1 • [m13]=10 nm • SA:biotin staple 15:1 • this annealing run was not dialyzed before adding SA and imaging

  3. Two biotin on each side of A tile SA labeled A tile 58.88nm

  4. SA labeled 2D cross DNA origami array

  5. below is a flowchart type key to the AFM images in the subsequent slides, any images that are not square can be assumed to have long side (X) length equal to the Y range seen on side of image. Unless otherwise stated, all images on a single .ppt slide are of the very SAME mica surface/depostion. Also, unless stated, deposition was by 5 min. incubation of origami soln. on mica followed by single HOH rinse. A subunit with total 4 biotin B subunit with no biotin A subunit with total 4 biotin B subunit with no biotin + + + 15:1 SA:biotin staples anneal over weekend anneal over weekend AB array X AFM #1 (mica) AB array YSA AB array X 66nM SA (15:1) + 66nM SA (15:1) + AB array YSA 4 deg C overnight 4 deg C overnight AFM #2 (mica) AB array XSA AB array YSASA All AFM images shown in final 6 slides of this presentation were previously shown in group meeting by Massud and are of AB array XSA

  6. AFM #1 AB array YSA first mica after one HOH rinse

  7. AFM #1 AB array YSA same mica after second HOH rinse

  8. AFM #2 AB array YSASA first mica after one HOH rinse

  9. AFM #2 AB array YSASA second mica one HOH rinse

  10. AB array X AFM #1

  11. AB array XSA AFM #2 this is the first piece of mica imaged, TAE rinsed at left, TAE rinse followed by HOH rinse below

  12. AB array XSA AFM #2 second piece mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; continued next slide

  13. same mica surface/ deposition as seen in previous slide

  14. AB array XSA AFM #2 second peice mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; the only difference between this slide and the 2 preceding slides is that the same mica surface seen in previous 2 slides, sat in covered dish in ambient conditions for 6 days prior to acquisition of the images on this slide

  15. Massud included the following slides in his 6-21-11 presentation

  16. SA labeled 2D cross DNA origami array (this and all slides following are of AB array XSA)

  17. SA labeled 2D cross DNA origami array

  18. SA labeled 2D cross DNA origami array

  19. SA labeled 2D cross DNA origami array

  20. SA labeled 2D cross DNA origami array

  21. SA labeled 2D cross DNA origami array

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