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MICROBIOLOGIC DX.

3 FORMS : ANTIGENS ANTIBODIES SUGESTIVES TESTS. MICROBIOLOGIC DX. ANTIGENS. MICROSCOPIC OBSERVATION salin solution a) wet prep dark field indian ink. Ags. gram stain acid fast stain b) fix prep giemsa

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MICROBIOLOGIC DX.

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  1. 3 FORMS : ANTIGENS ANTIBODIES SUGESTIVES TESTS MICROBIOLOGIC DX.

  2. ANTIGENS • MICROSCOPIC OBSERVATION • salin solution • a) wet prep dark field • indian ink

  3. Ags. • gram stain • acid fast stain • b) fix prep giemsa • hematoxilin • argentic stain

  4. Ags.: Bacterian identification. • A) cultures: B.A., EMB, SS, • LOWENSTEIN • B) tissues cultures • C) animal inoculations

  5. ags • Molecular studies: • DNA, probes, Pcr, western-blot, chemioluminiscency

  6. antibodies • A) specific tests • B) inespecific tests

  7. ANTIBODIES • Meanings: has, had, o cross reaction. • fever reaction: Widal, Hudlesson • and Weil-Felix ASLO: ANTISTREPTOLISINS • VDRL: VENERAL DISEASE REACTION • LABORARATORY

  8. ABS • IG M: TOXOPLASMOSIS (SABIN –FELDMAN) IN NEW BORNE = INFECTION • IG G: TOXOPLASMOSIS IS MOTHER’S • IG G

  9. INESPECIFIC EXAMPLES • WEIL-FELIX • VDRL • EB TEST

  10. RUTIN TESTSGENERAL TESTS • C.B.C. RED CELLS • W.B.C. • INCREASE: ACUTE • Number bacterial INFECTION • NORMAL O DECREASE: • chronic INFECTION Platelets: decrease gram negative infection.

  11. WBC • DIFERENTIAL COUNT NEUTROPHILS INCREASE: ACUTE • INFECTION • LYNPHOCYTES INCREASE: CHRONIC o VIRAL INFECTION

  12. OTHERS LIKE: CSF o UA. • Pleocytosis (leucocytosis spinal fluid) = • infection. • PMN = ACUTE INF. • MN = CHRONIC or VIRAL INF. • Leucocyturia = UTI

  13. Clinical case • NURSE 30 YEARS OLD . SUPERVISOR • ( NIGHT ) “ RAMON GARIBAY “ HOSPITAL, FEVER ( 39 ) HEADACHE DURING 2 WEEKS . Fever reaction (+ )‏ • 1:640 (WEIL-FELIX) . TX . AMPICILIN

  14. Normal flora • 1.- Why flora = most of microorganisms are • bacterías. • 13 • 2.- How many cells are in the body?:10 • 14 • bacterias : 10

  15. flora • Skin, nose,mouth • intestinal, urogenital

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