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MICROBIOLOGY Practical Class

MICROBIOLOGY Practical Class. FOUNDATION BLOCK (2018). Dr . Malak M. El-Hazmi. MICROBIOLOGY. Laboratory diagnosis of infectious diseases. Microscopic examination. culture. Serological tests ( Ab ). Detection of Ag. Molecular method. Types of specimens. Bacteriology. Gram Stain.

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MICROBIOLOGY Practical Class

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  1. MICROBIOLOGYPractical Class FOUNDATION BLOCK (2018) Dr . Malak M. El-Hazmi

  2. MICROBIOLOGY

  3. Laboratory diagnosis of infectious diseases • Microscopic examination. • culture. • Serological tests (Ab). • Detection of Ag. • Molecular method .

  4. Types of specimens

  5. Bacteriology

  6. Gram Stain G- bacilli

  7. Bacterial cell wall

  8. Bacterial Shapes and Arrangements

  9. Gram Stain

  10. GRAMPOSITIVEBACTERIA

  11. GRAMPOSITIVECOCCI

  12. GRAMPOSITIVECOCCI

  13. GRAM NEGATIVE BACTERIA

  14. Gram negative cocci (Diplococci ) e.gNeisseria • Gram negative bacilli • e.g E. coli • Salmonella

  15. Gram positive cocci in chain Streptococci Gram positive cocci in clusters Staphylococci Rx cloxacillin Cephalosporin if MRSA vancomycin Penicillin Cephalosporin

  16. A gram-stained smear of a CSF sample from a 3 year old child seen in the emergency department presenting with fever and neck stiffness. Gram-positive diplococci & pus cells Streptococcus pneumoniae

  17. This is a bacterium isolated from a child with sore throat and tonsillitis . A: Describe the Gram stain Gram positive B: Describe the shape and arrangement of the bacteria Cocci in chains

  18. Following is the Gram stained smear of an organism isolated from a wound infection. Describe what you see in the slide above. Gram-positive cocci in clusters What is the likely organism ? Staphylococcus aureus

  19. Following is the Gram-stained smear of from urethra of a 25 –year old male complaining of urethral discharge Describe the Gram stain of the intracellular bacteria Gram negative Describe the shape of the bacteria cocci ( diplococci)

  20. Describe the Gram stain of this organism: Gram negative Describe its shape bacilli ( rods )

  21. Bacterial Culture Media

  22. Bacterial Culture Media

  23. Bacteria culturing 1-Inoculation 3-Incubation 2-streaking Laboratory Incubator

  24. Identification of streptococci by hemolytic reaction Beta-hemolytic Streptococcus colonies Alpha-hemolytic Streptococcus colonies Gamma-hemolytic Streptococcus colonies

  25. GRAM NEGATIVE BACTERIA

  26. MacConkey's agar (Deferential medium)

  27. Biochemical testings • To confirm • the identity • of bacteria. Antibiotic susceptibility testings

  28. Automated instrument for identification and susceptibility testings

  29. Blood agar This is a general culture medium used for culture of bacteria.

  30. This is a blood agar growing beta hemolytic streptococci.

  31. This culture was grown from a sputum specimen of a 60 year old man complaining of cough, fever and chest pain. α hemolytic streptococci on blood agar

  32. VIROLOGY

  33. Viral structure

  34. Viral Classification

  35. Viral Electron Micrographs

  36. Herpes simplex virus -1 : Herpesviridae Enveloped virus Icosahedral capsid d.s DNA genome Loose envelope

  37. These are electron micrographs of a virus Herpes virus Q1: Name this virus Enveloped virus , Icosahedral capsid, d.s DNA genome Q2: Describe its structure.

  38. Adenovirus : Adenoviridae Nonenveloped virus Icosahedral capsid d.s DNA genome Only V with fiber

  39. This is an electron micrograph of a virus Adenovirus Q1: Name this virus • Nonenveloped virus, with fiber • Icosahedral capsid & d.s DNA genome Q2: Describe its structure.

  40. Rabies virus: Rhabdoviridae Enveloped virus Helical capsid s.s RNA genome Bullet shape

  41. This is an electron micrograph of a virus Q1: Name this virus • Rabies virus Enveloped virus , Helical capsid & s.s RNA genome Q2: Describe its structure.

  42. Influenza Viruses : Orthomyxoviridae Enveloped V & spikes Helical capsid Segmented s.s RNA Pleomorphic shape

  43. This is an electron micrograph of a virus Q1: Name this virus Influenza Viruses Enveloped Virus with spikes , Helical capsid ,Segmented s.s RNA Q2: Describe its structure

  44. parasitology

  45. Classification of Parasites

  46. Ascarislumbricoides (roundworm) Ascaris adult

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