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Biofilms

Biofilms. Dental plaque. Problems associated to biofilms. 1- Physical effects: ships, tubing…. 2- Metal surface corrosion . 3- Development on medical implants. 4- Associated diseases. Tubing obstruction. Equipamiento dental >30años. M +. MS 2-. Biofilm formation on a voice implant.

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Biofilms

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  1. Biofilms

  2. Dental plaque

  3. Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants 4- Associated diseases

  4. Tubing obstruction Equipamiento dental >30años

  5. M+ MS2-

  6. Biofilm formation on a voice implant

  7. Contact lens

  8. Catheter

  9. P. Aeruginosa biofilm in lung

  10. Structure

  11. Structure

  12. Biofilm formation process

  13. Glass surface in the lab

  14. Genetic analysis of biofilm formation WT LBF MBF HBF Citrate Glucose+Caa

  15. Genetic characterization of biofilm formation - Requirement of movement - Adhesins -Signaling -Glycocalyx formation

  16. Movement requirement • Flagelar-mediated mobility • Non-motile mutants in P. Fluorescens • Twitching • Mutants in type IV pili in P. aeruginosa • Suppression by cells “stickier” • ompR and adhesin Curli in E. coli

  17. Adhesins • Type I pilus (fimH) in E. coli • Type IV (MSHA) pilus in V. cholerae • Curli adhesin

  18. Signaling • Quorum sensing

  19. QUORUM SENSING:DENSITY-DEPENDENT EXPRESSION TRESHOLD AUTOINDUCER CULTURE DENSITY

  20. GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE LuxR LuxR LuxI Target gene

  21. GRAM-NEGATIVE COMMUNICATION: THE LuxI/LuxR LANGUAGE • Light production in Vibrio fisheri • Virulence in Pseudomonas aeruginosa (LasI/R, RhlI/R) • Opine formation in Agrobacterium tumefaciens (TraR/I)

  22. GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE H ABC D Pheromone gene Target gene

  23. HYBRID LANGUAGES: THE CASE OF Vibrio harvey LuxQ LuxN LuxLM LuxS LuxO Target gene

  24. GRAM-positive COMMUNICATION: THE PHEROMONE LANGUAGE • Competence for DNA uptake in Bacillus subtilis and Streptoccocus pneumoniae (ComX/ComP/ComA) • Sporulation in Bacillus subtilis • Conjugation in Enteroccus fecalis • Virulence in Staphylococus areus

  25. QS-mutants

  26. Glycocalyx formation • Alginate production in P. aeruginosa • EPS in V. cholerae (vps)

  27. lasI rhl vps Curli fimA fimH alg flagella

  28. Natural process of biofilm formation / Control by a blocking analogue

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