1 / 11

Lecture 1 Introduction to recombinant DNA Technology

Lecture 1 Introduction to recombinant DNA Technology. Dr Muhammad Imran. What is a gene?. Gene is a piece of DNA which encode an RNA molecule which may encode a protein. What is a genetic engineering?.

mavis
Télécharger la présentation

Lecture 1 Introduction to recombinant DNA Technology

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Lecture 1Introduction to recombinant DNA Technology Dr Muhammad Imran

  2. What is a gene? • Gene is a piece of DNA which encode an RNA molecule which may encode a protein What is a genetic engineering? • Set of techniques by which one can deliberatelyinsert new piece/s of DNA into the existing DNA piece to modify the characters of an organism. Gene Cloning • Set of experiments carried out to create a recombinant molecule and its propagation in an organism/host organism multiplication.

  3. PCR: Polymerase Chain Reaction • A reaction in which we use DNA polymerase to make the copies of fragment of DNA selectively amplified with the help of primers

  4. History of rDNA Technology GregorMendel1850s and 1860s the birth of genetics

  5. What genes are and how they work • W. Sutton…the factors (genes) reside on Chromosomes 1903. • TH Morgan ……. Endorsed Sutton…..and gene mapping started in 1910 and by 1922 nearly 2000 genes were mapped. • Set of experiments by Avery, MacLeod, and McCarty in 1944, and of Hershey and Chase in 1952 proved that DNA is hereditary material and not the proteins

  6. 1952-1966 well-done Watson and Crick • Structure of DNA was elucidated, genetic code cracked, and the processes of • transcription and translation described Anticlimax era and frustration in late 1960 1971–1973 recombinant DNA technology or genetic engineering Gene cloning Kary Mullis discovered a revolutionary technique now called PCR

  7. Gene Cloning T.A Brown 6th Edition

  8. Properties to DNA and its replication • DNA is double helix • Double helix is anti-parallel • Replication only takes place from 5-3 • Replication is semi conservative • Replication is bidirectional

  9. PCR: Polymerase Chain Reaction • Quite different from gene cloning • Very simple • Easy to do less time consuming • Economical • Wide application

  10. Principle of Primer designing • Few things to be considered while designing the primers

  11. Continued………………..

More Related