1 / 9

SURVEY OF BIOCHEMISTRY Nucleic Acids continued… Amino Acids

SURVEY OF BIOCHEMISTRY Nucleic Acids continued… Amino Acids. PRS Question. Name the following compound ATP UTP GTP CTP. PRS Question. Thymine has a methyl group at which position?. C1 C3 C5 C1’. Last Week…. Recombinant DNA Technology Vectors, Cloning and Libraries

mia-schultz
Télécharger la présentation

SURVEY OF BIOCHEMISTRY Nucleic Acids continued… Amino Acids

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SURVEY OF BIOCHEMISTRYNucleic Acids continued…Amino Acids

  2. PRS Question • Name the following compound • ATP • UTP • GTP • CTP

  3. PRS Question • Thymine has a methyl group at which position? • C1 • C3 • C5 • C1’

  4. Last Week… • Recombinant DNA Technology • Vectors, Cloning and Libraries • Genomic vs. cDNA Library • How are libraries made? • PCR (for making copies of DNA) • How does PCR work? • What is required to conduct PCR?

  5. Reference: Wikipedia Genomic Library • Cut Genomic DNA with restriction enzymes • Insert fragments into vectors • Culture cells

  6. Making cDNA Image from Biochemistry by Stryer

  7. Making cDNA Clones Image from Biochemistryby Stryer

  8. 3 steps for each PCR cycle • Each PCR cycle includes: • A denaturation step (92-96oC) separates the two DNA strands. • A primer annealing step (40-75oC) which is a few degrees below the Tm of the primers. • A primer extension step (72oC) which is the optimal temperature for Taq DNA polymerase activity.

  9. Components for PCR KNOW THIS!! • Heat-stable DNA polymerase Thermus aquaticus Taq polymerase • Primers (DNA oligonucleotides) • Deoxynucleotides –dATP, dTTP, dCTP, dGTP • DNA template • Mg++, buffer components, and water

More Related