THEATRE

1. Sono state prese in esame le schede allergologiche e diagnostiche di undici cantanti lirici di cui 5 donne (2 soprano e 3 mezzosoprano) e 6 maschi ( 3 tenori, 2 baritoni e 1 basso) di eta’ media di 43 anni( +8.92 rangee30-61) che presentarono a partire dal 2002 rinocongiuntivite ed asma prevalentemente nel periodo invernale e precoce–primaverile e che si riesacerbava durante la permanenza nei camerini e nelle sale prova del Teatro Lirico Comunale di Bologna. Cupressaceae pollenosis in lyrical singers in Bologna,Italy. D. Cagnetti°, L. Forlani ° AUSL città di Bologna; °° Dipartimento di Biologia evoluzionistica Sperimentale - Università di Bologna Istanbul (Turchia) 11-15 june 2011 - EAACI A small percentage of professional lyrical solo performers and chorus members (11 in total) involved with the Teatro Comunale of Bologna showed winter/early-spring seasonal respiratory problems of allergic etiology derived from trees’pollen. Symptoms were evident while practicing in the rehearsal venues located behind the theatre and facing a green area which included a public park, a private garden and several plants. Such upper and lower respiratory pathways disease reduced singing performance ability affecting professional voice users. GARDEN cypress cause THEATRE In order to identify the main cause of such pollinosis, a portable volumetric bio-aerosol pollen-trap was placed on a balcony of the theatre, facing those green areas: results showed a high concentration of Cupressaceae pollen originated from 2 undersized cypress trees in the private garden (“neighbourhood pollenosis”) Pollentrap All singers had winter/early-spring rhino-conjuntivitis, 4 with asthma, 7 with contact dermatitis, 10 with several comorbilitys,positive in vivo and in vitro SPT 3+/RAST 3-4°) to Cupressaceae; 2 to Graminaceae, 8 to mites, 3 to mouldes, 9 to food,6 to contact substances.All singers received 5/6 daily medicines like steroids, beta2-agonists, antileucotrienes, antistamines; 3 of them had already been vaccinated 2 for mites and 1 for graminaceae. Collected singers data were compared with a control group equally allergic to Cupressaceae ,noticing substantial differences regarding high risk of exposure,lifestyle, work-related consequences and overall quality of life consequence LYRICAL SINGERS Aerobiological monitoring and clinical correlation have shown high environmental impact of cause-effect for professional voice users and diagnostic accuracy highlighting the current allergic magnitude of Cupressaceae pollen Due to the current impact of Cupressaceaepollenosis, several epidemiological studies have been conducted, the preparation of valid diagnostics tests and effective specific immunotherapies both through injection and sublingual subministration were achieved Given our best knowledge, through improvements of patient education and better information flow from non-medical local professionals (botanists-architects- city planners-politics ) future scenarios should embrace “pollen-free” green spaces

2. Sono state prese in esame le schede allergologiche e diagnostiche di undici cantanti lirici di cui 5 donne (2 soprano e 3 mezzosoprano) e 6 maschi ( 3 tenori, 2 baritoni e 1 basso) di eta’ media di 43 anni( +8.92 rangee30-61) che presentarono a partire dal 2002 rinocongiuntivite ed asma prevalentemente nel periodo invernale e precoce–primaverile e che si riesacerbava durante la permanenza nei camerini e nelle sale prova del Teatro Lirico Comunale di Bologna. Cupressaceae pollenosis in lyrical singers in Bologna,Italy. D. Cagnetti°, L. Forlani ° AUSL città di Bologna; °° Dipartimento di Biologia evoluzionistica Sperimentale - Università di Bologna Istanbul (Turchia) 11-15 june 2011 - EAACI A small percentage of professional lyrical solo performers and chorus members (11 in total) involved with the Teatro Comunale of Bologna showed winter/early-spring seasonal respiratory problems of allergic etiology derived from trees’pollen. Symptoms were evident while practicing in the rehearsal venues located behind the theatre and facing a green area which included a public park, a private garden and several plants. Such upper and lower respiratory pathways disease reduced singing performance ability affecting professional voice users. GARDEN cypress cause THEATRE In order to identify the main cause of such pollinosis, a portable volumetric bio-aerosol pollen-trap was placed on a balcony of the theatre, facing those green areas: results showed a high concentration of Cupressaceae pollen originated from 2 undersized cypress trees in the private garden (“neighbourhood pollenosis”) Pollentrap All singers had winter/early-spring rhino-conjuntivitis, 4 with asthma, 7 with contact dermatitis, 10 with several comorbilitys,positive in vivo and in vitro SPT 3+/RAST 3-4°) to Cupressaceae; 2 to Graminaceae, 8 to mites, 3 to mouldes, 9 to food,6 to contact substances.All singers received 5/6 daily medicines like steroids, beta2-agonists, antileucotrienes, antistamines; 3 of them had already been vaccinated 2 for mites and 1 for graminaceae. Collected singers data were compared with a control group equally allergic to Cupressaceae ,noticing substantial differences regarding high risk of exposure,lifestyle, work-related consequences and overall quality of life consequence LYRICAL SINGERS Aerobiological monitoring and clinical correlation have shown high environmental impact of cause-effect for professional voice users and diagnostic accuracy highlighting the current allergic magnitude of Cupressaceae pollen Due to the current impact of Cupressaceaepollenosis, several epidemiological studies have been conducted, the preparation of valid diagnostics tests and effective specific immunotherapies both through injection and sublingual subministration were achieved Given our best knowledge, through improvements of patient education and better information flow from non-medical local professionals (botanists-architects- city planners-politics ) future scenarios should embrace “pollen-free” green spaces

3. Sono state prese in esame le schede allergologiche e diagnostiche di undici cantanti lirici di cui 5 donne (2 soprano e 3 mezzosoprano) e 6 maschi ( 3 tenori, 2 baritoni e 1 basso) di eta’ media di 43 anni( +8.92 rangee30-61) che presentarono a partire dal 2002 rinocongiuntivite ed asma prevalentemente nel periodo invernale e precoce–primaverile e che si riesacerbava durante la permanenza nei camerini e nelle sale prova del Teatro Lirico Comunale di Bologna. Cupressaceae pollenosis in lyrical singers in Bologna,Italy. D. Cagnetti°, L. Forlani ° AUSL città di Bologna; °° Dipartimento di Biologia evoluzionistica Sperimentale - Università di Bologna Istanbul (Turchia) 11-15 june 2011 - EAACI A small percentage of professional lyrical solo performers and chorus members (11 in total) involved with the Teatro Comunale of Bologna showed winter/early-spring seasonal respiratory problems of allergic etiology derived from trees’pollen. Symptoms were evident while practicing in the rehearsal venues located behind the theatre and facing a green area which included a public park, a private garden and several plants. Such upper and lower respiratory pathways disease reduced singing performance ability affecting professional voice users. GARDEN cypress cause THEATRE In order to identify the main cause of such pollinosis, a portable volumetric bio-aerosol pollen-trap was placed on a balcony of the theatre, facing those green areas: results showed a high concentration of Cupressaceae pollen originated from 2 undersized cypress trees in the private garden (“neighbourhood pollenosis”) Pollentrap All singers had winter/early-spring rhino-conjuntivitis, 4 with asthma, 7 with contact dermatitis, 10 with several comorbilitys,positive in vivo and in vitro SPT 3+/RAST 3-4°) to Cupressaceae; 2 to Graminaceae, 8 to mites, 3 to mouldes, 9 to food,6 to contact substances.All singers received 5/6 daily medicines like steroids, beta2-agonists, antileucotrienes, antistamines; 3 of them had already been vaccinated 2 for mites and 1 for graminaceae. Collected singers data were compared with a control group equally allergic to Cupressaceae ,noticing substantial differences regarding high risk of exposure,lifestyle, work-related consequences and overall quality of life consequence LYRICAL SINGERS Aerobiological monitoring and clinical correlation have shown high environmental impact of cause-effect for professional voice users and diagnostic accuracy highlighting the current allergic magnitude of Cupressaceae pollen Due to the current impact of Cupressaceaepollenosis, several epidemiological studies have been conducted, the preparation of valid diagnostics tests and effective specific immunotherapies both through injection and sublingual subministration were achieved Given our best knowledge, through improvements of patient education and better information flow from non-medical local professionals (botanists-architects- city planners-politics ) future scenarios should embrace “pollen-free” green spaces

4. Sono state prese in esame le schede allergologiche e diagnostiche di undici cantanti lirici di cui 5 donne (2 soprano e 3 mezzosoprano) e 6 maschi ( 3 tenori, 2 baritoni e 1 basso) di eta’ media di 43 anni( +8.92 rangee30-61) che presentarono a partire dal 2002 rinocongiuntivite ed asma prevalentemente nel periodo invernale e precoce–primaverile e che si riesacerbava durante la permanenza nei camerini e nelle sale prova del Teatro Lirico Comunale di Bologna. Cupressaceae pollenosis in lyrical singers in Bologna,Italy. D. Cagnetti°, L. Forlani ° AUSL città di Bologna; °° Dipartimento di Biologia evoluzionistica Sperimentale - Università di Bologna Istanbul (Turchia) 11-15 june 2011 - EAACI A small percentage of professional lyrical solo performers and chorus members (11 in total) involved with the Teatro Comunale of Bologna showed winter/early-spring seasonal respiratory problems of allergic etiology derived from trees’pollen. Symptoms were evident while practicing in the rehearsal venues located behind the theatre and facing a green area which included a public park, a private garden and several plants. Such upper and lower respiratory pathways disease reduced singing performance ability affecting professional voice users. GARDEN cypress cause THEATRE In order to identify the main cause of such pollinosis, a portable volumetric bio-aerosol pollen-trap was placed on a balcony of the theatre, facing those green areas: results showed a high concentration of Cupressaceae pollen originated from 2 undersized cypress trees in the private garden (“neighbourhood pollenosis”) Pollentrap All singers had winter/early-spring rhino-conjuntivitis, 4 with asthma, 7 with contact dermatitis, 10 with several comorbilitys,positive in vivo and in vitro SPT 3+/RAST 3-4°) to Cupressaceae; 2 to Graminaceae, 8 to mites, 3 to mouldes, 9 to food,6 to contact substances.All singers received 5/6 daily medicines like steroids, beta2-agonists, antileucotrienes, antistamines; 3 of them had already been vaccinated 2 for mites and 1 for graminaceae. Collected singers data were compared with a control group equally allergic to Cupressaceae ,noticing substantial differences regarding high risk of exposure,lifestyle, work-related consequences and overall quality of life consequence LYRICAL SINGERS Aerobiological monitoring and clinical correlation have shown high environmental impact of cause-effect for professional voice users and diagnostic accuracy highlighting the current allergic magnitude of Cupressaceae pollen Due to the current impact of Cupressaceaepollenosis, several epidemiological studies have been conducted, the preparation of valid diagnostics tests and effective specific immunotherapies both through injection and sublingual subministration were achieved Given our best knowledge, through improvements of patient education and better information flow from non-medical local professionals (botanists-architects- city planners-politics ) future scenarios should embrace “pollen-free” green spaces

5. Sono state prese in esame le schede allergologiche e diagnostiche di undici cantanti lirici di cui 5 donne (2 soprano e 3 mezzosoprano) e 6 maschi ( 3 tenori, 2 baritoni e 1 basso) di eta’ media di 43 anni( +8.92 rangee30-61) che presentarono a partire dal 2002 rinocongiuntivite ed asma prevalentemente nel periodo invernale e precoce–primaverile e che si riesacerbava durante la permanenza nei camerini e nelle sale prova del Teatro Lirico Comunale di Bologna. Cupressaceae pollenosis in lyrical singers in Bologna,Italy. D. Cagnetti°, L. Forlani ° AUSL città di Bologna; °° Dipartimento di Biologia evoluzionistica Sperimentale - Università di Bologna Istanbul (Turchia) 11-15 june 2011 - EAACI A small percentage of professional lyrical solo performers and chorus members (11 in total) involved with the Teatro Comunale of Bologna showed winter/early-spring seasonal respiratory problems of allergic etiology derived from trees’pollen. Symptoms were evident while practicing in the rehearsal venues located behind the theatre and facing a green area which included a public park, a private garden and several plants. Such upper and lower respiratory pathways disease reduced singing performance ability affecting professional voice users. GARDEN cypress cause THEATRE In order to identify the main cause of such pollinosis, a portable volumetric bio-aerosol pollen-trap was placed on a balcony of the theatre, facing those green areas: results showed a high concentration of Cupressaceae pollen originated from 2 undersized cypress trees in the private garden (“neighbourhood pollenosis”) Pollentrap All singers had winter/early-spring rhino-conjuntivitis, 4 with asthma, 7 with contact dermatitis, 10 with several comorbilitys,positive in vivo and in vitro SPT 3+/RAST 3-4°) to Cupressaceae; 2 to Graminaceae, 8 to mites, 3 to mouldes, 9 to food,6 to contact substances.All singers received 5/6 daily medicines like steroids, beta2-agonists, antileucotrienes, antistamines; 3 of them had already been vaccinated 2 for mites and 1 for graminaceae. Collected singers data were compared with a control group equally allergic to Cupressaceae ,noticing substantial differences regarding high risk of exposure,lifestyle, work-related consequences and overall quality of life consequence LYRICAL SINGERS Aerobiological monitoring and clinical correlation have shown high environmental impact of cause-effect for professional voice users and diagnostic accuracy highlighting the current allergic magnitude of Cupressaceae pollen Due to the current impact of Cupressaceaepollenosis, several epidemiological studies have been conducted, the preparation of valid diagnostics tests and effective specific immunotherapies both through injection and sublingual subministration were achieved Given our best knowledge, through improvements of patient education and better information flow from non-medical local professionals (botanists-architects- city planners-politics ) future scenarios should embrace “pollen-free” green spaces