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attR4

attL1. Reporter. attL2. attL4. Promoter. attR1. attR2. Terminator. attL3. Promoter Entry Clone. Reporter Entry Clone. Terminator Entry Clone. +. +. Kan R. Kan R. Kan R. +. attR4. Cm R - ccdB. attR3. Destination Vector (Amp R Cm R ccdB + ). Amp R. LR clonase. attB4.

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attR4

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  1. attL1 Reporter attL2 attL4 Promoter attR1 attR2 Terminator attL3 Promoter Entry Clone Reporter Entry Clone Terminator Entry Clone + + KanR KanR KanR + attR4 CmR-ccdB attR3 Destination Vector (AmpR CmR ccdB+) AmpR LR clonase attB4 Promoter attB1 Reporter attB2 Terminator attB3 Expression Clone (AmpR) AmpR + attP4 CmR-ccdB attP3 Plasmid By-product kanR attP1 kanR attP2 kanR

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