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B IOMATERIALS L AB N ETWORK D EGRADATION

B IOMATERIALS L AB N ETWORK D EGRADATION. Monday, February 6 th and Tuesday, February 7 th. P RE-LAB. Sign-in Quiz (Metters. 2000). Rectangular Approximation.

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B IOMATERIALS L AB N ETWORK D EGRADATION

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  1. BIOMATERIALS LABNETWORKDEGRADATION Monday, February 6th and Tuesday, February 7th

  2. PRE-LAB • Sign-in • Quiz (Metters. 2000)

  3. Rectangular Approximation "Approximating the Area Under a Curve for AP Calculus | Education.com." Education.com | An Education & Child Development Site for Parents | Parenting & Educational Resource. Web. 06 Feb. 2012. <http://www.education.com/study-help/article/rectangular-approximations/>.

  4. Procedural Notes • Teamwork • Take turns pipetting, massing, ect. but make sure you remember what has been added to the solution • Pipetting • Check the volume, change by twisting dial • When filling, go to first stop • When ejecting precursors, go to second stop • When ejecting into syringe mold, go to first stop (to avoid bubbles)

  5. LINEAR MOLECULES • PEG-PLA-MA • MSA MAA (CH2)4

  6. METHACRYLATE POLYMERIZATION Step-growth Polymerization 1 “Arm” …… PEG PLA MA Unlike last lab, each double bond can hook up with TWO other molecules. (Lin, C.C., Pharmaceutical Research. 26(3): 631-643. 2009)

  7. SECTION 1 PEG-PLA-MA Network Synthesis • Can surmise from Metters.2000 this will be bulk degrading • Monitor change in network over time… • Size (diameter / height) • Weight • Young’s Modulus • Stereo-microscopy Imaging Polyanhydrides. Wikipedia. 2011

  8. PEG NETWORK SYNTHESIS • 10wt% PEG-PLA-MA in PBS • 10% photo-initiator by volume (LAP) • 40μL placed in 1mL syringe (tip cut off) • to create cylindrical hydrogel sample geometry • exposed to UV-lamp for 10min • Remove and place in PBS • 3 PEG samples

  9. SECTION 2 MSA/MAA Network Synthesis • Glassy polymer • Monitor change in network over time… • Size (diameter / height) • Weight • Young’s Modulus • Stereo-microscopy Imaging Polyanhydrides. Wikipedia. 2011 (CH2)4

  10. MSA/MAA NETWORK SYNTHESIS Unlike PEG-PLA-MA, the MSA/MAA is a bulk reaction that is not polymerized in a liquid phase. • ≈0.25g of MSA/MAA • 0.1wt% DMAP photo-initiator added to PA-MA • Fill mold “sandwich” (glass slide / Teflon / glass slide) • exposed to UV-lamp for 10min • Remove and place in PBS • 1 MSA/MAA sample

  11. TESTING • Young’s modulus in Materials Testing Lab – 5N load cell • Mass – after patting networks dry • Size – use calipers for height and diameter. Don’t squish hydrogel • All three will be assembled and posted on blackboard. • Stereo-microscopy Imaging – collect images with digital camera • Each group is responsible for collecting their own images • All performed on D0, D1, D3, D8, D10

  12. Big Picture • Control of mechanical properties & degradation behavior allows tuning to your application • Ex: Control degradation rate  Control drug release rate • Bulk • Typically hydrophilic  Biologically inert • Large number of available chemistries • Surface • Typically hydrophobic  Protein adsorption, cell interaction • Constant turnover at surface  difficult to integrate with tissue • Applications • Prevent postsurgical adhesion formation • In vivo drug and protein delivery • Temporary scaffold for tissue repair • Degradable sutures

  13. SECTION 3 Degradation time-course • Look at how networks degrade over time • 1 team member per data collection day

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