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Epigenetica e Cancro

Epigenetica e Cancro. Pier Mario Biava Istituto di Ricovero e Cura a Carattere Scientifico Multimedica Milano. MOLECULAR WEIGHT OF DIFFERENT PROTEINS PRESENT IN ZEBRAFISH EMBRYO DURING GASTRULATION PERIOD. 1) 37% molecular weight of about 97KDa 2) 14.6% molecular weight of about 45 KDa

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Epigenetica e Cancro

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  1. Epigenetica e Cancro Pier Mario Biava Istituto di Ricovero e Cura a Carattere Scientifico Multimedica Milano

  2. MOLECULAR WEIGHT OF DIFFERENT PROTEINS PRESENT IN ZEBRAFISH EMBRYO DURING GASTRULATION PERIOD 1) 37% molecular weight of about 97KDa 2) 14.6% molecular weight of about 45 KDa 3+4) 27.4% molecular weight of about 30-25 KDa 5) 4% molecular weight of about 20 KDa 6+7) 14% molecular weight of about 14 KDa

  3. Continues

  4. Spiral CT during the arterial phase prior to treatment withstem cell differentiation stage factors(SCDSF) shows an advanced HCC of the right lobe. Neoplastic hypervascularized areas are present in segment 7, and a hypervascularized thrombus (arrow) occupies the right portal branch and reaches the main trunk.

  5. In the same patient, power doppler confirms the thrombosis inside the main portal vein (asterisk)

  6. Spiral CT performed 6 months after treatment with SCDSF shows the shrinkage of portal thrombus and disappearance of HCC in the right lobe

  7. Power doppler confirms the shrinkage of the thrombosis and the patency of the main portal vein

  8. In another case, CT during arterial phase prior to treatment with SCDSF shows several nodules of HCC

  9. CT performed 6 months after treatment with SCDSF shows the disappearance of neoplastic hypervascularization inside the nodules

  10. Treatment with stem cell differentiation stage factors of intermediate-advanced HCC: an open randomized clinical trial. 100 80 60 Survival (%) 40 20 Objective response & stable disease Progression 0 0 6 12 18 24 30 36 Months Livraghi T, Meloni F, Frosi A et al. Oncol Res 2005

  11. Analisi delle proteine degli estratti di zebrafish: 5 fasi di sviluppo (ZF1-ZF5) e una mix delle 5 fasi (ZF6) SDS Page degli estratti embrionali di Zebrafish

  12. Saggio di proliferazione: estratti di zebrafish: 6 fasi (ZF1-ZF6) a 4 concentrazioni, dopo 24 ore di trattamento Saggio di proliferazione

  13. Saggio di proliferazione: estratti di zebrafish: 6 fasi (ZF1-ZF6) a 4 concentrazioni, dopo 72 ore di trattamento Saggio di proliferazione p < 0,05 p < 0,01

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