1 / 14

Fibroblast Growth Factor 3 (FGF3) int-2

Fibroblast Growth Factor 3 (FGF3) int-2. Brandon Williams. 3/25/03. OBJECTIVES. Biochemical Information Normal Function in Mouse Ear Development Breast Cancer and FGF3. EASY FACTS. 22 FGFs in vertebrates FGF3: Chromosome 11 (humans) Fgf3: Chromosome 7 (mice)

neo
Télécharger la présentation

Fibroblast Growth Factor 3 (FGF3) int-2

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fibroblast Growth Factor 3 (FGF3)int-2 Brandon Williams 3/25/03

  2. OBJECTIVES • Biochemical Information • Normal Function in Mouse Ear Development • Breast Cancer and FGF3

  3. EASY FACTS • 22 FGFs in vertebrates • FGF3: Chromosome 11 (humans) • Fgf3: Chromosome 7 (mice) • Are secreted polypeptides • Use tyrosine kinase receptors for signaling • Proto-oncogene

  4. FUNCTIONS • Cell proliferation, migration, and differentiation during embryonic development • Tissue repair, injury response in adults • Angiogenesis

  5. FGF and Tyrosine Kinases • FGFs bind to heparan sulfate and tyrosine kinase receptors (FGFRs) • FGF binds to heparan sulfate • FGF/heparan sulfate dimer binds to a FGFR • Complexes form dimers • Signal transduction

  6. FGFRs • 7 prototypes from 4 genes • 2 or 3 immunoglobulin-like extracellular domains • Heparan sulfate binding domain • Activated through autophosphorylation • Signaling pathway includes Ras, Raf, SOS, etc.

  7. Receptor Dimerization

  8. FGFRs

  9. What Does FGF3 do in a wt Mouse? • Mouse lives (viable) • Inner ear problems • Skeletal problems (tail)

  10. Mouse Ear Development • FGF3 was thought to induce inner ear development • McKay et al. showed that FGF3 is required for correct development of inner ear, not induction of otic placode

  11. Kreisler Mutant Mice • Cannot be maintained as completely homozygous line (females do not breed and repeated outcrossing is needed to produce breeding stud males) • Develop a otic placode etc. • Inner ear is mutant

  12. FGF Expression

  13. Breast Cancer Numbers • Most frequent cancer among women, other than skin cancer • 203,500 new cases per year in U.S. • 40,000 die each year • 1 in 8 women • 5% attributed to mutated BRCA1

  14. FGF3 and Breast Cancer • Mouse Mammary Tumor Virus (MMTV) • Insertion of MMTV activates several genes including FGF3 that have been associated with breast cancer • Mixed results • Cyclin D is closely linked to FGF3

More Related