1 / 11

Sequencing Using DNA dependant DNA polymerase

*. dATP. dATP. dCTP. ddCTP. DNA template. dTTP. dATP. ddCTP. dGTP. dGTP. *. *. *. dCTP. dTTP. dATP. Denature & anneal labelled primer. Sequencing Using DNA dependant DNA polymerase. Initiation & elongation commences. ddCTP. *. ddCTP. DNA template. ddCTP.

nickan
Télécharger la présentation

Sequencing Using DNA dependant DNA polymerase

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. * dATP dATP dCTP ddCTP DNA template dTTP dATP ddCTP dGTP dGTP * * * dCTP dTTP dATP Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Initiation & elongation commences ddCTP

  2. * ddCTP DNA template ddCTP Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dTTP dATP dGTP dGTP * dTTP dATP * *

  3. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Elongation terminated by dideoxyCTP dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP dATP * * ddCTP

  4. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Elongation continues for remaining molecules dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP dATP * * ddCTP

  5. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP * * ddCTP

  6. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * dTTP * * ddCTP

  7. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * dTTP * * ddCTP

  8. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * * * ddCTP

  9. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP dATP dATP ddCTP dCTP dATP dGTP * ddCTP * * ddCTP

  10. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP dATP dATP dCTP dATP dGTP * ddCTP * ddCTP * ddCTP

  11. Sequencing Using DNA dependant DNA polymerase • Multiple products of different length terminated by A dideoxyCTP • Theses molecules represent all the positiions within the complementary sequence where a “C” occurs • Four separate reactions each terminated by a different dideoxy molecule would represent all bases • Ordering these molecules by size allows us to determine the template sequence * ddCTP * ddCTP * ddCTP * ddCTP * ddCTP * * ddCTP ddCTP

More Related