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Solutions & Dilutions

Solutions & Dilutions. Intro to Spectrophotometry. SOLUTIONS. DILUTIONS. Solute Solvent. Simple dilution Serial dilutions. 1 can juice 4 cans water 1:5 dilution factor. Serial Dilution. Spectrophotometry. Light has: A wave nature which gives rise to the visible spectrum of light

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Solutions & Dilutions

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  1. Solutions & Dilutions Intro to Spectrophotometry

  2. SOLUTIONS DILUTIONS • Solute • Solvent • Simple dilution • Serial dilutions 1 can juice 4 cans water 1:5 dilution factor

  3. Serial Dilution

  4. Spectrophotometry • Light has: • A wave nature which gives rise to the visible spectrum of light • A particle nature which gives rise to the photoelectric effect (used in the spec.)

  5. Light Waves

  6. When Light Strikes Matter…

  7. White Light Blue Light The red end of the spectrum has been absorbed Blue light has been transmitted

  8. Color Wheel

  9. Isolating Wavelengths

  10. Design of a Spectrophotometer

  11. Photoelectric Effect

  12. Light Transmission Dependence on Concentration Beer’s Law

  13. Cuvettes & The Blank

  14. Standardization Graph

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