GPCR Edition – Week 1

GPCR Edition – Week 1

Amino Acid Structure Amino Acids: Misc. GPCR Structure Chemical Interactions Protein Secondary & Tertiary Structure Potpourri 100 100 100 100 100 100 200 200 200 200 200 200 300 300 300 300 300 300 400 400 400 400 400 400 500 500 500 500 500 500

Glycine

Valine

Cysteine

Histidine

Tryptophan

The amino acid arginine has this grouping of amino acids, along with lycine and histidine (if the pH is just right) Positively charged

This amino acid can be abbreviated as “Gln” or “Q” Glutamine

The amino acid arginine is abbreviated as “Arg” as well as this one letter R

“alpha” refers to this element that is covalently bonded to an amino group, a carboxyl group, a hydrogen, and a side chain Carbon

This number is the net negative or positive charge of the following short oligopeptide: DEKHR +1

GPCRs are also known as hetpahelical receptors, meaning they have this number of alpha helices seven

GPCRs are embedded into a membrane bilayer composed of this type of biological molecule Phospholipid

GPCRs are very difficult to crystallize and often times have crystallization “handles” fused to them. This one is similar to what we used in our experiment. T4 Lysozyme

This class of GPCRs has a large extracellular domain with a “venus-flytrap” structure where ligands bind Class C

Class A GPCRs have this conserved sequence motif of three amino acids that mediates coupling to G proteins DRY, Asp-Arg-Tyr

The attraction of a cation to an anion is referred to as this type of non-covalent chemical interaction Ionic/Electrostatic

The non-covalent interaction shown here Hydrogen Bond

This non-covalent is what drives non-polar amino acids to orient themselves towards the interior of a protein and away from water Hydrophobic Effect

A peptide bond is a covalent chemical interaction between the amino group of one amino acid and this group of the other Carboxyl

DAILY DOUBLE Hydrogen bonding between the carbonyl oxygen and amino group hydrogen of two amino acids 4 residues apart stabilizes this type of structure Alpha helix

This is the simplest arrangement a polypeptide chain can make in terms of secondary structure Alpha Helix

This amino acid’s rigidity results in its low probability of being found in an alpha helix Proline

The partial positive and negative charge of an alpha helix N- and C-terminus, respectively, is referred to as this Helix Dipole

This is the name of the chart that measures the probability of particular psi and phi angles to be found in secondary structure Ramachandran

This protein structural motif is named after a common architectural design found in the Hellenic Republic Greek Key Motif

This level of protein structure is the arrangement of 2 or more separate polypeptide chains (subunits) Quaternary

GPCRs are composed primarily of this type of secondary structure Alpha Helix

The functional and folded structure of a protein in its normal environment is known as this Native conformation

The formation of a peptide bond between two amino acids results in the creation of this molecule, which is why the reaction is called condensation Water

Which university is the only one in NCAA history to hold the national title in both basketball and football? University of Florida

GPCR Edition – Week 1