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Progress report 13.06.05

Progress report 13.06.05. RESULTS and FUTURE ACTIVITIES. FOTON experiments: growth curves. P. putida in FOTON medium (defined M9Glu medium supplemented with 20% LB medium) at 30°C (shaking); inoculum 10 6 cfu/ml. Results similar for other bacteria.

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Progress report 13.06.05

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  1. Progress report 13.06.05 RESULTS and FUTURE ACTIVITIES

  2. FOTON experiments: growth curves P. putida in FOTON medium (defined M9Glu medium supplemented with 20% LB medium) at 30°C (shaking); inoculum 106 cfu/ml. Results similar for other bacteria

  3. Scheme of the FOTON-experiment: different materials and species used

  4. Planned experiments The experiment from the FOTON flight will be replicated on Earth following the indications of the temperature data logger Adhesion on material will be determined either by staining and microscopic analysis or by quantitative FISH; space and Earth samples will be compared Fluorescence staining of a steel coupon colonized by P. putida

  5. Adhesion by mixed bacterial communities Experiments performed in the first part of the project point to synergistic biofilm formation by mixed bacterial populations

  6. Setting up new (and old) methods to measure adhesion/biofilm formation 30°C 37°C Fmed. Fmed. LB LB 1 2 3 4 5 6 1 2 3 4 5 6 Curli-producing strain Fimbriae-producing strain

  7. A new test: cell aggregation Sedimentation due to cell aggregation Reduced sedimentation in non-aggregative strains (Experiment by E. Brombacher; protocol as in Simm et al., 2004)

  8. Apologies for not being able to be here today…. • See you in Milan in November!

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