Bacteriological Monitoring Community-Based Watershed Stewardship in Kenya

1. Bacteriological Monitoring Community-Based Watershed Stewardship in Kenya This workshop is conducted by the Green Belt Movement and Global Water Watch, as part of a project funded by the U.S. EPA in Cooperative Agreement with the Institute for Governance and Sustainable Development.

2. 1. What is Global Water Watch (GWW) and how did they partner with the Green Belt Movement?

3. Water Facts • 1.2 billion people have no access to safe and • adequate water supplies • 3 billion people do not have adequate sanitation • 1 million deaths/year from waterborne infectious disease • … mostly children • 80% of all disease in developing countries is due to contaminated water and poor sanitation • Epidemic cholera reappeared in Latin America after a 90-year absence…now in 20 countries! • The poor pay the most for water, and suffer the most from water-related problems

4. 2. What is the GWW-GBM Project in Community-Based Water Monitoring?

5. 3. How can community members (non-specialists) collect good scientific information?

7. 4. What do GWW volunteers monitor?

8. 5. What is a watershed?

9. 6. How does the flow and purity of rivers and springs relate to tree-planting and land cover?

10. 7. Why is it important to monitor bacteria in water?

11. 8. What are bacteria and where do they live?

12. 9. What kinds of benefits and problems do bacteria cause?

13. Campylobacter Shigella Listeria

14. 10. Why is the bacterium, E. coli of special interest?

15. 11. How are E. coli related to other bacteria?

16. 12. Where does E. coli come from?

17. 13. How does E. coli get into water and what does this indicate?

18. 14. What are the characteristics of a good water monitoring site?

19. 15. Why do we determine the GIS data (geo-reference) for each sampling site?

20. 16. How can we test water for E. coli?

21. 17. What are the parts of the test kit for E. coli?

22. 18. How is each part of the sample kit used and labeled?

23. 19. How is the dropper used to collect a good water sample?

25. 20. What is in the sample bottle and how does it indicate the presence of E. coli?

26. 21. How does the plate work and why does the medium become solid when poured into the plate?

27. 22. What is done with the water sample after it is put into the plate?

28. 23. Why and how are E. coli samples incubated?

29. 24. What happens to the E. coli when the sample is incubated? 29 - 37  24–48 hours

30. 25. How is the number of E. coli determined from the incubated plates?

31. Determine Results Reading, counting and interpreting bacteriological plates

32. Counting Exercise • E. Coli = E/V x 100 • E. Coli = 7/1 x 100 • E. Coli = 7 x 100 • E. Coli = 700

33. Cleanup

34. 26. What amount of E. coli in water is safe for human contact and drinking?

35. 27. How can the E. coli data teach us about what is happening in a watershed?

36. Community Watershed Map on Floor

37. Plates on Map with Data Sheets

38. Monitors Analyze Their Samples

39. Disturbing Trends! 40 42 38

40. Encouraging Trends! 40 42 38

41. 28. How can we use this information to improve watersheds and drinking water quality?

42. 29. How can we share this information with our family, friends, neighbors, educators, students, government officials, and the public?

43. 30. What are the next steps for the GWW-GBM project and community involvement?

44. Global Water Watch and Green Belt Movement Project Community-Based Watershed Stewardship in Kenya Bacteriological Monitoring Workshop March 2014