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Center for Homogeneous DNA Analysis - Software

Center for Homogeneous DNA Analysis - Software. April 21, 2005. Second Year Advances. Background removal Melting curve calculators Automatic clustering and classification. Background removal. Superior to baseline method Enables correct classification of unlabeled probe melting curves

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Center for Homogeneous DNA Analysis - Software

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  1. Center for Homogeneous DNA Analysis - Software April 21, 2005

  2. Second Year Advances • Background removal • Melting curve calculators • Automatic clustering and classification

  3. Background removal • Superior to baseline method • Enables correct classification of unlabeled probe melting curves • Makes HLA matching, repeat typing and sequencing more accurate • Improves determination of thermodynamic parameters, and therefore all PCR design and analysis tools • In use by ARUP Labs

  4. Melting curve calculators • More informative than just Tm calculator • Enhances design of full genotyping studies • Improves determination of thermodynamic parameters

  5. Automatic clustering and classification algorithms • Used in HLA identity testing, mutation scanning, and genotyping

  6. WWW availability • Center for Homogeneous DNA analysis • Homepage: www.dna.utah.edu • DNAWizards beta site by remote panels • URL: DNAWizards.path.utah.edu • Small amplicon primer design: SNPWizard • Melting curve calculators: Melt1.0, Het1.0

  7. The coming year • Determine a complete set of Tm Parameters • superior background removal • realistic experimental conditions (dyes, salt) • mismatched duplexes (synthetic) • Genome wide primer promiscuity prevention • Further develop user-friendly suite of programs • Commercial launch of DNAWizards.com

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