1 / 6

Shigella spp Isolation and Serotyping

Shigella spp Isolation and Serotyping. Shigella spp. Shigella is a group of bacteria that has four different species that cause gastrointestinal illness. Group A - Shigella dysenteriae Group B - Shigella flexneri Group C - Shigella boydii Group D - Shigella sonnei.

osanna
Télécharger la présentation

Shigella spp Isolation and Serotyping

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Shigella spp Isolation and Serotyping

  2. Shigella spp • Shigella is a group of bacteria that has four different species that cause gastrointestinal illness. • Group A - Shigella dysenteriaeGroup B - Shigella flexneriGroup C - Shigella boydiiGroup D - Shigella sonnei

  3. Shigella spp Isolation • Shigella organisms may be very difficult to distinguish biochemically from Escherichia coli. • Shigella species are Gram-negative, facultatively anaerobic, nonsporulating, nonmotile rods in the family Enterobacteriaceae. • They do not decarboxylate lysine or ferment lactose within 2 days. NON-LACTOSE FERMENTERS: COLOURLESS COLONIES MacConkey Agar LACTOSE FERMENTERS: RED/PINK COLONIES

  4. Identification bacterial pathogens in stool by biochemical testing Non-lactose fermenting, morphologically different, and well-isolated Colony from SS and/ or Mac. 1. Inoculate the Kliger’s Iron Agar (KIA) agar tube by touching the top of a colony using sterilized inculcation needle and stabbing to the bottom of the KIA. After stabbing, immediately streak the slanted surface of the agar. 2. Inoculate the Motility-Indole-Ornithine (MIO) tube by stabbing, in a single up-and-down needle. 3. Inoculate the Lysine-Iron agar (LIA) by stabbing the butt to the bottom once and streaking the slant. 4. Inoculate the urea broth tube by placing the needle in the tube and shaking the needle. 5. Incubate all tubes overnight at 35-37 Co Conventional biochemical screening sets

  5. Each serologic group containsspecific or major Somatic Antigen (O Ag) Characteristic of this group Each serologic group is subdivided on the basis of antigenic structure into Serotypes  Serotypes is further subdivided into subserotypes on the basis of antigenic structure   Serogroup Serotypes Subserotypes Serogroups and Serotypes

  6. Serogroups and Serotypes are determined by visual inspection of slide agglutination assays, with commercial antisera used as described by the manufacture.

More Related