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Fig. S1

HincII. HincII. XbaI. XbaI. Wild type allele. Probe. Probe. Targeting vector. Neo. XbaI. XbaI. Targeted allele. Neo. p53. +/+. +/+. +/-. +/-. -/-. -/-. mkk7. mkk7. MDM2. +/-. +/-. -/-. -/-. p-ERK. +/-. +/-. -/-. -/-. p-p38. +/-. +/-. -/-. -/-.

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Fig. S1

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  1. HincII HincII XbaI XbaI Wild type allele Probe Probe Targeting vector Neo XbaI XbaI Targeted allele Neo p53 +/+ +/+ +/- +/- -/- -/- mkk7 mkk7 MDM2 +/- +/- -/- -/- p-ERK +/- +/- -/- -/- p-p38 +/- +/- -/- -/- Whole embryos x 5 Liver x 200 +/- +/- -/- -/- a Fig. S1 b c Kbp mkk7 neo allele 15 wild type allele 12 actin d Penninger

  2. Fig. S2 mkk7 +/- mkk7 -/- 0 h 0 h H2O2, 48h H2O2, 48h G1: 59% S: 10% G2/M 31% G1: 67% S: 14% G2/M 19% G1: 70% S: 12% G2/M 18% G1: 55% S: 7.8% G2/M 37% mkk7 +/- mkk7 -/- 40 30 The full scan for Fig. 2a G2/M (%) 20 TNFa UV 10 -/- mkk7 +/+ +/- +/+ +/- -/- +/+ +/- -/- mkk7 +/- -/- -/- 0 mkk4 - - + 0 48 0 48 GST-c-Jun 86 (h) after H2O2 69 pBabe mkk7 (kDa) 52 mkk7-/- mkk7+/+ mkk7 +/+ +/- -/- +/+ +/- -/- MKK4 121 40 86 184 control control control control pBabe mkk7 mkk7 121 69 26 52 86 121 69 JNK 86 40 (kDa) 69 52 184 26 MKK7 52 40 121 40 86 26 69 26 (kDa) (kDa) 52 a-actin MKK7 The full scan for Fig. 2c JNK 40 The full scan for Fig. 3b 26 The full scan for Fig. 2d a b c d e f Penninger

  3. Fig. S3 mkk7+/- mkk7-/- 1 4 7 10 1 4 7 10 121 mkk7 +/+ +/- -/- 86 184 61 121 49 mkk7 +/+ +/- -/- 86 36 Cdc2 69 184 32 121 52 MKK4 121 86 86 69 61 40 mkk7 +/+ +/- -/- +/+ +/- -/- mkk7 49 p53 52 CyclinD1 184 36 184 121 184 32 40 121 86 184 121 86 61 36 121 ATF-2 69 86 32 86 26 52 49 c-Jun 61 69 p21 20 40 36 Jun D 15 52 49 JNK 32 40 +/- -/- mkk7 +/- -/- +/- -/- +/- 26 -/- 121 121 p16 20 mkk7 86 86 +/+ +/- -/- mkk7 +/+ +/- -/- 184 15 69 86 61 61 The full scan for Fig. 4b 121 GST- c-Jun 184 61 86 121 49 49 HH1 Cdc2 CycB1 52 49 p-c-Jun 86 61 26 36 61 40 26 49 49 MAPK Jun B 36 28 26 mkk7+/- mkk7-/- Passage No. 3 6 8 3 6 8 mkk7 +/+ +/- -/- 121 cdc2 mkk7 mkk7 mkk7 mkk7 +/+ +/- -/- +/+ +/+ +/+ +/- +/- +/- -/- -/- -/- 86 61 49 p38 26 28S mkk7 +/+ +/- -/- 18S 121 86 61 49 36 Cdc2 32 a The full scan for Fig. 4a b c d The full scan for Fig. 4c JNK The full scan for Fig. 4d Penninger

  4. Fig. S4 mkk7 -/- -/- +/- +/- mkk7 2 5 mkk7-/- Passages c-Jun AA -/- AA -/- +/+ +/+ Control 121 c-Jun 86 +/- -/- +/- -/- actin 61 49 49 cdc2 36 36 Cdc2 hgf Cdc2 met 32 32 61 2 5 Passages c-Jun -/- +/- +/- -/- AA -/- AA -/- +/+ +/+ 49 184 Actin 121 121 86 86 36 61 61 49 JNK GST- c-Jun 49 26 P-c-Jun 36 32 mkk7+/+ mkk7-/- control c-Jun pBabe control 121 86 61 c-Jun 49 36 32 121 86 61 49 Actin 36 32 a b c The full scan for Fig. 4g The full scan for Fig. 4f The full scan for Fig. 6a d f e wt JunAA 4 6 8 4 6 8 Passages cdc2 28S 18S The full scan for Fig. 6c The full scan for Fig. 6b The full scan for Fig. 7d Penninger

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