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1 2 3 4 5 6 7 8 9. Actin HO-1. 1. MCF7 control 7h 2. MCF7 0.5uM AW464 7h 3. MCF7 1uM AW464 7h 4. MCF7 5uM AW464 7h 5. MCF7 control 16h 6. MCF7 0.5uM AW464 16h 7. MCF7 1uM AW464 16h 8. MCF7 5uM AW464 16h 9. MCF7 menadione 3.5h 100uM. 1 2 3 4 5 6 7 8 9.

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  1. 1 2 3 4 5 6 7 8 9 Actin HO-1 1. MCF7 control 7h 2. MCF7 0.5uM AW464 7h 3. MCF7 1uM AW464 7h 4. MCF7 5uM AW464 7h 5. MCF7 control 16h 6. MCF7 0.5uM AW464 16h 7. MCF7 1uM AW464 16h 8. MCF7 5uM AW464 16h 9. MCF7 menadione 3.5h 100uM

  2. 1 2 3 4 5 6 7 8 9 1. MCF7 control 3h 2. MCF7 AW464 0.5uM 3h 3. MCF7 AW464 1uM 3h 4. MCF7 AW464 5uM 3h 5. MCF7 control16h 6. MCF7 control16h (loaded twice in error) 7. MCF7 AW464 0.5uM 16h 8. MCF7 AW464 1uM 16h 9. MCF7 AW464 5uM 16h

  3. 1 2 3 4 5 6 7 8 9 1. MCF7 control 24h 2. MCF7 AW464 0.5uM 24h 3. MCF7 AW464 1uM 24h 4. MCF7 AW464 5uM 24h 5. marker lane 6. 100uM H2O2 1h MCF7 7. 1mM H2O2 1h MCF7 8. 100uM H2O2 4h MCF7 9.1mM H2O2 4h MCF7

  4. Primary Ab: HO-1 SantaCruz mouse monoclonal 2ug/ml in 5% marvel Secondary: Goat anti-mouse-HRP conjugated, Pierce 1/4000 in 1% marvel Cells grown in 10cm petri dishes and harvested by scraping in lysis buffer on ice. Loading:15ug per well 1 2 3 4 5 6 7 8 9 1. HCT116 H2O2 1 mM 15 min 2. HCT116 control 3h 3. HCT116 0.5 uM AW464 3h 4. HCT116 1 uM AW464 3h 5. HCT116 5 uM AW464 3h 6. HCT116 control 7h 7. HCT116 0.5 uM AW464 7h 8. HCT116 1 uM AW464 7h 9. HCT116 5 uM AW464 7h

  5. 1 2 3 4 5 6 7 8 9 1. HCT116 H2O2 100uM 30min 2. HCT116 control 16h 3. HCT116 0.5 uM AW464 16h 4. HCT116 1 uM AW464 16h 5. HCT116 5 uM AW464 16h 6. HCT116 control 24h 7. HCT116 0.5 uM AW464 24h 8. HCT116 1 uM AW464 24h 9. HCT116 5 uM AW464 24h

  6. 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 Same blot, different exposures. 1. HCT116 control 16h 2. HCT116 0.5uM AW464 16h 3. HCT116 1uM AW464 16h 4. HCT116 5uM AW464 16h 5. HCT116 control 24h 6. HCT116 0.5uM AW464 24h 7. HCT116 1uM AW464 24h 8. HCT116 5uM AW464 24h 9. HCT116 5FU 10ug/ml 24 h uneven loading problem apparent (actin bands)

  7. 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 Same blot, different exposures. 1. HCT116 control 3h 2. HCT116 0.5uM AW464 3h 3. HCT116 1uM AW464 3h 4. HCT116 5uM AW464 3h 5. HCT116 control 7h 6. HCT116 0.5uM AW464 7h 7. HCT116 1uM AW464 7h 8. HCT116 5uM AW464 7h 9. H2O2 1mM 4h uneven loading problem apparent (actin bands)

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