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Cytoskeleton System

Chapter 8. Cytoskeleton System. Biology Department of the Basic Teaching Colledge. Xiamixinuer · Yilike. March of 2012. Teaching Requirements:. 1. Mastering: concepts of the cytoskeleton; structure, chemical composition, and assembly of microtubules and microfilaments.

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Cytoskeleton System

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  1. Chapter 8 CytoskeletonSystem Biology Department of the Basic Teaching Colledge Xiamixinuer·Yilike March of 2012

  2. Teaching Requirements: 1. Mastering: concepts of the cytoskeleton; structure, chemical composition, and assembly of microtubules and microfilaments. 2. Comprehending: functions of microtubules and microfilaments. 3. Understanding: functions of the cytoskeleton; types and functions of intermediate filaments.

  3. The cytoskeleton

  4. 1. Introduction A. Conception of Cytoskeleton (Narrow sense) A complex network of interconnected microfilaments, microtubules and intermediate filaments that extends throughout the cytosol.

  5. The comparison among three types of the cytoskeleton

  6. Fluorescent microscopy and Electron microscopy : • Immunofluorescence: fluorescently-labeled antibody • Fluorescence: microinject into living cells • Video microscopy: in vitro motility assays • Electron: Triton X-100, Metal replica • Drugs and mutations (about functions) • Biochemical analysis(in vitro) B. Techniques for studying the cytoskeleton

  7. Fluorescence microscopy microtubules actin filamin cytoskeleton microfilaments microtubules microtubules

  8. C. The self-assembly and dynamic structure of cytoskeletal filaments • Each type of cytoskeletal filament is constructed from smaller protein subunits. • The cytoskeleton is a network of three filamentous structures. • The cytoskeleton is a dynamic structure with many roles.

  9. D. The function of the cytoskeleton • Structural support • Internal framework maintaining position of the organelles • Machinery required for movement of materials and organelles within cells • Force generating elements responsible for movement of cells from one place to another

  10. 2.Microtubule, MT A. Structures: • Hollow • Tubular structures 25nm in diameter • Assembled from protein tubulin • The tubulin consists of alpha-beta tubulin heterodimers arranged in rows (protofilaments) • Form cytoskeleton, mitotic spindle, centrioles, core of cilia and flagella

  11. a and ßTubulin heterodimers are the protein building blocks of MTs

  12. Arrangement of protofilaments in singlet, doublet, and triplet MTs Singlet Doublet Triplet A A B B C In cilia and flagella In centrioles and basal bodies

  13.                                                                                                                                                                                                                                                                                  Assembling process of MT OUTSIDE OF THE BODY + PEDAL -

  14. assemble Head tail connection 2 3 1 4 13 5 12 6 11 7 10 9 8 CROSSSECTION                          tubulin heterodimer MT (13) profilament  tubulin  

  15. B. MTs assemble from microtubule-organizing centers (MTOCs) Microtubule-organizing centers (MTOCs):is the region to assemble MT,Where includes-tubulin. MTOCs:includeCentrosome, Mitotic spindle and Basal body.

  16. (1) Interphase: Centrosome Microtubule-organizing centers (MTOCs) Dynamic instability (2) Dividing cell: Mitotic spindle Dynamic instability (3) Ciliated cell: Basal body Stability

  17. Centrosome is a microtubule organizing center, MTOCs

  18. Centrosome containing a pair of centrioles

  19. Centrioles Centrioles are short cylinders with a 9 + 0 pattern of microtubule triplets. Centrioles may be involved in microtubule formation and disassembly during cell division and in the organization of cilia and flagella.

  20. MT are nucleated by a protein complex containing -tubulin The centrosome is the major MTOC of animal cells

  21. Why the centrosome can act as MTOC? Treat cell with colcemid Cytosolic MTs depoly, except those in centrosome A • Experiments supporting that centrosome is the MTOC Expla I: MTOC nucleate poly of tubulins Remove colcemid Tublin repoly Expla II: MTOC gather MTs in cytosol B centrosome + Tubulins MT + Tubulins No

  22. Cilia and flagella Cilia (small and numerous) and flagella (large and single) have a 9 + 2 pattern of microtubules and are involved in cell movement. Cilia and flagella move when the microtubule doublets slide past one another. Each cilium and flagellum has a basalbody at its base.

  23. Basal body structure

  24. Dynamic instability due to the structural differences between a growing and a shrinking microtubule end. • GTP cap; • Catastrophe: accidental loss of GTP cap; • Rescue: regain of GTP cap C. Characteristics of MT assembly

  25. Microtubules have a plus and minus ends. • Typically the minus is for anchoring and the plus is for growing. • The transition between MT growth and MT shrinking is controlled in cells by special proteins..

  26. Drugs affect the assembly of MTs (1) Colchicine Binding to tubulin dimers, prevent MTs polymerization (2)Taxol Binding to MTs, stabilize MTs These compounds are called antimitotic drugs, and have application in medical practice as anticancer drugs

  27. MAPs modulate MT structure, assembly, and function D. Microtuble-associated proteins (MAPs) Control organization Katanin like proteins MAPs Tau: In axon, cause MTs to form tight bundles MAP2: In dendrites, cause MTs to form looser bundles MAP1B: In both axons and dendrites to form crossbridge between microtubules

  28. MAP2 associated with brain MTs

  29. 5. Functions of MTs • A. Maintenance of cell shape(constitute the centriols and cilia or flagella). • B. Cell motility (see in cilia or flagella). • C. Chromosome movements in cell division • D. Organelle movement (MT associated motor proteins: kinesins: towards + end (anterograde transport) Golgi to ER or PM traffic;dyneins: towards - end (retrograde transport) ER to Golgi traffic.)

  30. 5. Functions of MTs A. Maintenance of cell shape(constitute the centriols and cilia or flagella).

  31. Constitute the centriols and cilia or flagella • No centrioles in Plant and fungi • A pair of centrioles are surrounded by electron dense pericentriolar material. • Centrioles contain nine evenly spaced fibrils, each containing three microtubules, A, B and C tubules. • A tubule is connected to the center of the centriole by a radial spoke. • Centrioles are in pairs and at right angles to each other. Structure

  32. Constitute the centriols and cilia or flagella

  33. 5. Functions of MTs • B. Cell motility (see in cilia or flagella).

  34. A comparison of the beating of flagella and cilia

  35. Ultrastructure of a eukaryotic flagellum or cilium

  36. Motility of MT(CILIA,FILAGELA MOVEMENT) SPERM MOVEMENT CILIA MOVEMENT

  37. Motor molecules and the cytoskeleton

  38. Motor molecules and the organelle

  39. Dyenin arms responsible for sliding Crosslinks and spokes responsible for bending

  40. B. Transport in the cytoplasm MT associated motor proteins: kinesins: towards + end (anterograde transport) Golgi to ER or PM traffic dyneins: towards - end (retrograde transport) ER to Golgi traffic

  41. C. Movement of chromosomes

  42. 3. Microfilament, MF • Using ATP, G-actin polymerizes to form MF(F-actin) A. MFs are made of actin and involved in cell motility.

  43. MINUS END F-actin G-actin PLUS END

  44. Assembly of MF -end G-actin Dimer Trimer +end F-actin

  45. Characteristics: (1) Within a MF, all the actin monomers are oriented in the same direction, so MF has a polarity B. MF assembly and disassembly Myosin is molecular motor for actins.

  46. (2) In vitro, (Polymerization) both ends of the MF grow, but the plus end faster than the minus. Because actin monomers tend to add to a filament’s plus end and leave from its minus end---- “Tread-milling”

  47. (3) Dynamic equilibrium between the G-actin and polymeric forms, which is regulated by ATP hydrolysis and G-actin concentration.

  48. (4) Dynamic equilibrium is required for the cell functions. Some MFs aretemporary and others permanent.

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