1 / 9

Buffers and its uses.

Buffers and its uses. Done By: Ng Sze Hian 1P1 (20). What is a buffer solution?. It is a solution that does not change the pH of a solution.

rianna
Télécharger la présentation

Buffers and its uses.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Buffers and its uses. Done By: Ng SzeHian 1P1 (20)

  2. What is a buffer solution? • It is a solution that does not change the pH of a solution. • Consists of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. It has the property that the pH of the solution changes very little when a small amount of strong acid or base is added to it. • Conjugate= (of an acid and a base) related by the loss or gain of a proton: NH 3  is a base conjugate to NH 4 + . NH 4 +  is an acid conjugate to NH 3 .

  3. Difference between a buffer solution, acidic buffer solution, and alkaline buffer solution. • A buffer solution is one which resists changes in pH when small quantities of an acid or an alkali are added to it. • An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt. • An alkaline buffer solution has a pH greater than 7. Alkaline buffer solutions are commonly made from a weak base and one of its salts.

  4. Examples of buffer solutions • One example of a buffer solution is blood as many life forms thrive only in a relatively small pH range.

  5. How do buffer solutions work? • A buffer solution has to contain things which will remove any hydrogen ions or hydroxide ions that you might add to it - otherwise the pH will change. Acidic and alkaline buffer solutions achieve this in different ways.

  6. Uses of buffer solutions • Our body uses buffers to maintain a blood pH of 7.35-7.45, and also in a massive number of biochemical reactions involving enzymes. • Used in biological experiments in the lab, especially those pertaining to proteins or DNA. • Buffer solutions are also widely used in industry. Industrial processes requiring buffer solutions include fermentation, controlling dye processes and manufacturing pharmaceuticals. • In research, buffers are used all the time to run biochemical assays. The pH must remain relatively constant for such assays, thus a buffer solution is used.

  7. Uses of buffer solutions… • It is also used in patch testing. Acid and alkaline products must usually be diluted before patch testing in order to be non-irritant. The use of ordinary solvents for the dilution often means low-concentration products to be tested. By using buffer solutions, the possible test concentrations can be increased at least many hundred fold. • In research, buffers are used all the time to run biochemical assays. The pH must remain relatively constant for such assays, thus a buffer solution is used.

  8. References • http://www.chemguide.co.uk/physical/acidbaseeqia/buffers.html • http://www.ehow.com/how-does_4912029_what-buffer-solutions-used.html

  9. THANK YOU!!!

More Related