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Inflammation Due To RA

Inflammation Due To RA. http://www.cedars-sinai.edu/Patients/Health-Conditions/Images/354031_Adv_Rheumatoid_Arthritis-2sm.jpg. Normal Joint. Bone. Normal Joint. Bone. Synovium. Normal Joint. Bone. Synovium. Cartilage. Rheumatoid Arthritis. Synovial Inflammation. Bone Destruction.

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Inflammation Due To RA

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  1. Inflammation Due To RA http://www.cedars-sinai.edu/Patients/Health-Conditions/Images/354031_Adv_Rheumatoid_Arthritis-2sm.jpg

  2. Normal Joint Bone

  3. Normal Joint Bone Synovium

  4. Normal Joint Bone Synovium Cartilage

  5. Rheumatoid Arthritis Synovial Inflammation Bone Destruction http://www.riversideonline.com/source/images/image_popup/mcdc7_arthritis_types.jpg

  6. Osteoclasts

  7. T helper Cells??

  8. Th17 Cells

  9. In Rheumatoid Arthritis,Do Th17 Cells play a major role in osteoclast differentiation in vivo?

  10. In Rheumatoid Arthritis,Do Th17 Cells play a major role in osteoclast differentiation in vivo? Can osteoclast differentiation be induced in vitro by Th17 via RANK/RANKL mechanism?

  11. Kim et al (2012)

  12. Kim et al (2012) RANKL

  13. Kim et al (2012) RANKL

  14. Kim et al (2012) Vitamin D RANKL

  15. Kim et al (2012) Vitamin D RANKL 3 FOLD

  16. Quantitative PCR After Reaction with RANKL

  17. Quantitative PCR After Reaction with RANKL RT-PCR / Sybr Green TRAP RNA GAPDH reference gene

  18. Since TRAP proteinis only produced in osteoclasts, it can be used to measure differentiation.

  19. Kim et al

  20. Kim et al

  21. Kim et al

  22. Kim et al

  23. Kim et al % of GAPDH

  24. Kim et al % of GAPDH Reduced Osteoclast Differentiation

  25. Kim et al Reduced RANK expression NO RANKL

  26. Kim et al Reduced RANK expression Reduction

  27. Proposed Experiment T helper Cells

  28. Proposed Experiment T helper Cells Vitamin D

  29. Can osteoclast differentiation be induced in vitro by Th17 via RANK/RANKL mechanism? • Th17 in synovium • Capable of inducing differentiation via RANKL/Rank • Kim et al showed that Vitamin D inhibits via RANK

  30. In Rheumatoid Arthritis,Do Th17 Cells play a major role in osteoclast differentiation in vivo? Use T cell specific RANKL knockout mice.

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