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PBIO 4500/5500: Biotechnology and Genetic Engineering

PBIO 4500/5500: Biotechnology and Genetic Engineering. Welcome! Complete the student information form. Tell the class about you. All the course information is on the course web site; I do not use Blackboard. Let’s go over the syllabus. Get the 5 th Edition of Molecular Biotechnology.

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PBIO 4500/5500: Biotechnology and Genetic Engineering

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  1. PBIO 4500/5500:Biotechnology and Genetic Engineering Welcome! Complete the student information form. Tell the class about you. All the course information is on the course web site; I do not use Blackboard. Let’s go over the syllabus. Get the 5th Edition of Molecular Biotechnology. Any questions?

  2. Chapter 1 - The Development of Molecular Biotechnology Molecular Biology/Biotechnology terminology Milestones in molecular biotechnology Model organisms for molecular/cellular biology Transcription and translation Prokaryotic gene organization & expression

  3. Terminology • Molecular biology-the study of biology on a molecular level including the structure, function, and makeup of biologically important molecules such as DNA, RNA, and proteins • Recombinant DNA technology-a set of techniques for manipulating DNA, including: the identification and cloning of genes; the study of the expression of cloned genes; and the production of large quantities of gene product • Genetic engineering-the process of transferring DNA from one organism into another that results in a genetic modification • Biotechnology-production of goods and services using biological organisms, systems, and processes • Molecular biotechnology-rDNA technology + biotechnology

  4. Table 1.1

  5. Table 1.1 (Continued)

  6. Many scientific disciplines contribute to molecular biotechnology, which generates a wide range of commercial products. Figure 1.1

  7. Model Systems for Molecular/Cellular Biologyhttps://www.dnalc.org/resources/animations/model_organisms.html • Viruses • Bacteria (E. coli) • Yeast (Saccharomyces cerevisiae) • Round worms (Caenorhabditis elegans) • Planarian (Schmidtea mediterranea) • Alga (Chlamydomonas reinhardtii) • Fruit fly (Drosophila melanogaster) • Zebrafish (Danio rerio) • Arabidopsis thaliana (thale cress) • Mouse (Mus musculus)

  8. Figure 1.13 Each model experimental organism used in molecular and cell biology has advantages for certain types of studies.

  9. Central Dogma of Biology transcription translation $$$ DNA RNA Protein reverse transcription Molecular Biotechnology DNA replication

  10. Chemical structure of DNA & RNA

  11. Chemical structure of dsDNA

  12. Chemical structure of dsDNA

  13. Prokaryotic Gene Structure and Expression

  14. In prokaryotes, RNA polymerase binds to the -10 and -35 regions of the promoter relative to the start site of transcription (+1) promoter operator

  15. Prokaryotic Gene Structure Eukaryotic Gene Structure Enhancers/ silencers

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