'Affinity chromatography' diaporamas de présentation

In the name of god

In the name of god. size exclusion (gel filtration, gel permeation ) chromatography. Introduction. Biomolecules are purified using chromatography techniques that separate them according to differences in their specific properties. Property Technique:

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Protein Methods

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27041, Week 02

27041, Week 02. Review of Week 01. The human genome sequencing project (HGP). Systems Biology and emergent properties. Different model representations. Chen et al., Mol. Biol. Cell., 2004. Model Generation. Systems Biology at a glance. YER001W YBR088C YOL007C YPL127C YNR009W YDR224C

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Protein Methods II

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Biochemistry for Bioseparation Engineering

Biochemistry for Bioseparation Engineering. 3-Dimensional Structure of Protein. Basic Structure of Amino Acid. Disulfide Bond. Peptide Bond. N- and C-terminals of Peptide. ???? ??. ???? ?? ??. Principles of Chromatography. Ion Exchange Chromatography. Gel Filtration Chromatography.

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Downstream Processing in Biopharmaceutical Manufacturing

Downstream Processing in Biopharmaceutical Manufacturing. Harvest and Clarification Tangential Flow Filtration (UF/DF) Low Pressure Liquid Column Chromatography. Know the Characteristics of Your Protein Human Serum Albumin. Sequence of Amino Acids. Tertiary Structure.

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Chapter 4. Exploring Proteins

Chapter 4. Exploring Proteins. I. The purification of proteins ( ?? ) 1. Differential Centrifugation : ??? ????? ?? ?? 2. Protein separation and purification 1) Salting-out ( ?? ) and dialysis ( ?? ): protein ???? ?? ?? 2) Chromatography( ?? ? ?? ):GFC, IEC, AC, HPLC

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Validation of Virus Removal

Validation of Virus Removal. Keith O.Webber, Ph.D. Deputy Director, Div. of Monoclonal Antibodies OTRR/CBER/FDA PDA/FDA Meeting September 26, 2000. SCOPE - Products. Specified Biologics Monoclonal Antibodies rDNA-derived products Traditional Biologics Some Blood-products

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PURIFICATION AND CHARACTERIZATION OF PROTEINS

PURIFICATION AND CHARACTERIZATION OF PROTEINS. BY ROHAN MENON. INTRODUCTION. Goals of purification vary with the intended use of the protein.

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Ion exchange chromatography

Chromatography for Protein purification 1. Ion-exchange chromatography 2. Hydrophobic chromatography 3. Affinity Chromatography 4. Size-exclusion / gel permeation chromatography. Ion exchange chromatography.

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Exploring Proteins and Proteomes

Exploring Proteins and Proteomes. Genomes and Proteomes. Obtaining the protein: cell lysis and Separation. Protein Purification Techniques. Salting out Dialysis Gel Filtration Chromatography Ion-exchange Chromatography Affinity Chromatography. HPLC Gel Electrophoresis

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Exploring Proteins and Proteomes

Exploring Proteins and Proteomes. Genomes and Proteomes. Obtaining the protein: cell lysis and Separation. Protein Purification Techniques. Salting out Dialysis Gel Filtration Chromatography Ion-exchange Chromatography Affinity Chromatography. HPLC Gel Electrophoresis

478 views • 25 slides

Separation t echniques

Separation t echniques. ?. Molecules can be separated : Chemically: b y charge, by action with specific reagents Physically: by solubility, by molecular weight, by density, structure (e.g. shape). Separation. 1. By physical properties. - Centrifugation (centrifugal force).

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Affinity Chromatography

Affinity Chromatography. Yongting Wang Jan07. What is AC?. Affinity chromatography (AC) is a technique enabling purification of a biomolecule with respect to biological function or individual chemical structure. AC is designed to purify a particular molecule from a mixed sample.

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Purification of chromosomal ParA1 protein for raising polyclonal antibodies

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IAC-SULF- Operation Manual by Clover Technology Group,Inc.

IAC-SULF- Operation Manual by Clover Technology Group,Inc. 1.0 IAC-SULF Test procedure for animal derived food overview. SAMPLE EXTRACTION Mix 5.0 g of muscle tissue homogenate and 20 ml ethanol/water(80:20,v/v) vortexed high speed for 2 minutes, and then shaken for 30min.

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Animal Cell Culture

Animal Cell Culture. Differences between procaryotes and eucaryotes. Cells are negatively charged . attach to positively charges surfaces some cells must attach to grow, others not examples of surfaces: sephadex, collagen

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BISC 220 Lab 2

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Phage Display and its Applications

Phage Display and its Applications. Matt Brown Human Genetics Dr. Nancy Bachman. Used for cloning foreign genes among other applications Proteins and peptides are fused to the Capsid (surface) of the phage The combination of the phage and peptide is known as a Fusion Protein. Basics.

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Localization and partial sequence of a postmolt cuticular glycosidase from Callinectes sapidus

Localization and partial sequence of a postmolt cuticular glycosidase from Callinectes sapidus Robert Roer, Thomas Shafer, David Towle & Kimberly Powell Dept. of Biological Sciences - University of NC at Wilmington & Mount Desert Island Biological Laboratory. NSF. ABSTRACT

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