'Arabinose operon' diaporamas de présentation

Regulation of Gene Expression

Regulation of Gene Expression. Information flow and regulatory factors. The number of copies of the gene The efficiency with which the gene is transcribed, which is mainly determined by the level of initiation of transcription by RNA polymerase (promoter activity). The stability of the mRNA

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Genetics of Bacteria

Genetics of Bacteria. Bacterial genome =. Genetics of Bacteria. Bacterial genome = One circular DNA molecule E. coli chromosome has 100 times more DNA than in a typical virus, but much less than a eukaryotic cell. Packed into nucleoid region of cell Plasmid =. Genetics of Bacteria.

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pGLO Transformation LAB AP LAB 6

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pGLO

pGLO. Bacterial Genetics. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. E. Coli structure. Plasmid. Additional DNA found in some bacteria Small loop Carries one or more genes

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1. Transform bacteria with pGlo plasmid 3. Induce expression of GFP with arabinose

Transformation Lab Creating a GMO by transforming E. coli. 1. Transform bacteria with pGlo plasmid 3. Induce expression of GFP with arabinose. 2. Select for ampicillin resistance. http://www.dnalc.org/harlemdnalab/ BacterialTrans.html. The recombinant pGlo plasmid.

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