'Differential media' diaporamas de présentation

Introduction to Microbiology

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bacterial Culture methods BASICS

bacterial Culture methods BASICS. Dr.T.V.Rao MD. Bacterial nutrition and the design of culture media. Based on bacterial metabolism* Culture pH Culture oxidation- reduction potential. Gaseous requirements Oxygen, Carbon dioxide and other gases.

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How Do Bacteria Reproduce?

How Do Bacteria Reproduce?. Not Mitosis Not Meiosis No Spindle Fibers Formed Prokaryotes Reproduce By Binary Fission. How Do Prokaryotes Reproduce?. The cell doubles in mass DNA replicates and the two strands separate

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Micro labs - review

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Lab 2:

Culture Media. Lab 2:. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any type of bacteria and the others are more selective and only grow specific types of bacteria. Types of Media.

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Bacteria Growth Characteristics

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Salmonella

Salmonella. Food poisoning; Enteric fever and Gastroenteritis. Bacteriology. Salmonella enterica one species, ~2000 serotype S. enterica serotype typhimurium or S. typhimurium Rod-shaped, non-spore-forming Gram-negative bacterium Belongs to the family Enterobacteriaceae

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Safety Notes

Safety Notes. Bunsen burners> open flame Tie long hair back NO microscopes out until Bunsen burners are off Safety glasses are to be worn at all times Must be worn until everyone is done at your table. BIO Safety. Work with opportunistic pathogens Must handle with extreme care

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Selective and Differential Media

Selective and Differential Media. Media for Isolation of Microbes. General (all purpose): contains basic nutrients for most bacteria Enriched : contains extra growth factors & nutrients (Fastidius organism)

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Introduction

Introduction. In addition to general-purpose media, which allow the growth of most types of bacteria, microbiologists use specialized media to identify and/or isolate specific groups of bacteria . Selective and differential media are used to isolate or identify particular organisms .

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Microbial growth requirements:

Microbial growth requirements:. Chemical growth factors: What are the chemical growth factors required for isolation of microbes in vitro ? 1-Carbon: organic: Glucose. Inorganic: CO 2 2- Hydrogen occurs in organic hydrocarbon molecules and in

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Culture media

Culture media. Media: Providing Nutrients in the Laboratory. At least 500 different types Contained in test tubes, flasks, or Petri dishes Inoculated by loops, needles, pipettes, and swabs Sterile technique necessary Classification of media Physical state Chemical composition

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The Composition of Bacteriological Media

The Composition of Bacteriological Media. Lab 4. Media preparation.

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Selective and Differential Media

Selective and Differential Media. Selective media Media designed to inhibit the growth of some organisms while encouraging the growth of others Differential media Media that contains substances (indicators) that reveal differences between organisms

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Selective and Differential Media

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Differential Media

Differential Media. Selective Media. Enriched Media. MacConkey Agar and EMB Agar. Streaking of Bacteria for Isolated Colonies.

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