'Facs analysis' diaporamas de présentation

ZFN WORK (JAN 2011- JAN 2013)

ZFN WORK (JAN 2011- JAN 2013). STANDARDIZATION TECHNIQUES. PCR RT PCR BLOT PI STAINING ANNEXIN V CEL-1/T7E1 ASSAY HOECHST ASSAY TRANSFECTION HIRT EXTRACTION. INITIAL EXPERIMENTS WITH SIGMA ZFN PAIRS. Transfection efficiency was good.

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F luorescence A ctivated C ell S orter

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Regulation of lck degradation and refractory state in CD8 + cytotoxic T lymphocytes

Regulation of lck degradation and refractory state in CD8 + cytotoxic T lymphocytes. Michael Uhlin, Maria G. Masucci, and Victor Levitsky * PNAS | June 28, 2005 | vol. 102 | no. 26 | 9264-9269. Date : 10/30/07 Name : Park Jong Chan. Abstract.

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[Supplementary materials]

[Supplementary materials]. Quantified high-throughput screening of Escherichia coli producing poly(3-hydroxybutyrate) based on FACS. Jae Hyung Lee, Seung Hwan Lee, Sung Sun Yim , Kyoung-Hee Kang, Sang Yup Lee, Si Jae Park, Ki Jun Jeong. a. b. c. d.

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Protein standard Whole cell lysate Cytosolic fraction Ni + -NTA affinity column

A. B. 120. 1. 2. 3. 4. 5. 6. prhTRAL. prhTRAL. c rhTRAL. c rhTRAL. 100. 80. Protein standard Whole cell lysate Cytosolic fraction Ni + -NTA affinity column Polymixin B Sepharose column PD-10 desalting column . 60. Cell viability (%). 40. 20. 0. 0. 50. 100. 150.

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DAPI LC3

Lysosensor Green DND-189. ESOM. CTR. DAPI LC3. A. B. EBSS. EBSS + E64d/PepA. 0 2h 4h 8h 2h 4h 8h. C. D. E. 0.33 0.27 0.57 0.79 0.73 1.04 1.47.

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FACS Staining Protocol

Each human cell expresses hundreds of thousands of cell surface antigens that specify their cell type, biological function, development stage, and much more. https://www.facs-analysis.com/flow-cytometry-protocols/

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Flow Cytometry Immunology

Each human cell expresses hundreds of thousands of cell surface antigens that specify their cell type, biological function, development stage, and much more. https://www.facs-analysis.com/flow-cytometry-protocols/

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FACS Protocols

Each human cell expresses hundreds of thousands of cell surface antigens that specify their cell type, biological function, development stage, and much more. https://www.facs-analysis.com/flow-cytometry-protocols/

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BMS

B. A. SK-N-SH cells, 24 h after irradiation. SK-N-SH cells, 48 h. 72 h after irradiation. 0 Gy. 5 Gy. Apo G1 G2/M. Apo G1 G2/M. Apo G1 G2/M. Apo G1 G2/M. 0 Gy. Cont. 2 76 9%. 2 71 17%.

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Results II Mice in vivo

Fig. 1. TLR2 and TLR4 are strongly up-regulated on both monocytes and neutrophils of patients (n=36) with severe melioidosis compared to healthy controls (n=32). Fig. 3. Reduced lung inflammation in TLR2 KO mice 72 hour after infection.

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Figure 1A.

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