'Gapdh' diaporamas de présentation

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synthetic nucleases: implications in hpv e6 gene editing

synthetic nucleases: implications in hpv e6 gene editing. FEB 2013- FEB 2014. PRELIMINARY WORK. 1 2 3 4. Lane 1: SiHa Lane 2: CasKi Lane 3: c33a Lane 4 : ladder. A). SiHa. SiHa. CasKi. E6 PCR. B). C). E6 sequence of SiHa and CasKi Cell line.

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CFTR

MP. MA. control. HQS. 200bp. CFTR. 400bp. GAPDH. A. MP. MA. control. HQS. 200bp. CFTR. GAPDH. 400bp. B. MP. MA. control. HQS. 200bp. CFTR. GAPDH. 400bp. C. MP. MA. control. HQS. 200bp. ?-ENAC. 400bp. GAPDH. G. MP. MA. control. HQS. 200bp. ?-ENaC. 400bp.

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Data and Question

Data and Question. Data: Time course data with two conditions (e.g. WT and mutant). For each condition: N time points and M replicates per time-point. In our example N =6, M =4. Every measurement represents a distinct mouse, there is no natural pairing across conditions.

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Supplementary figure 1

Supplementary figure 1. Supplementary figure 2 . sNF96.2. ST88-14. T265-2C. NF90.8. Supplementary figure 3. NF90.8. sNF96.2. T265-2C. ST88-14. Supplementary figure 4. A. B. NF90.8. siRNA mTOR#13. siRNA mTOR#19. siRNA GFP. mTOR. GAPDH. ST88-14. siRNA mTOR#13.

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Idenfitied Differentially Expressed Genes in Keratoconus

Idenfitied Differentially Expressed Genes in Keratoconus. Ji Eun Lee, Jong Soo Lee Department of Ophthlamology, Graduate School of Medicine, Pusan National University. Authors have no financial interest in any materials discussed in this article. Purpose.

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Untreated

Supplementary Figure 4. Untreated. 25 m M CQ. a. b. H460. T80. HEY. TOV112D. TOV21G. T80. 25 m M CQ. 25 m M CQ. 25 m M CQ. 25 m M CQ. 25 m M CQ. 12.5 m M CQ. 12.5 m M CQ. 12.5 m M CQ. 12.5 m M CQ. 12.5 m M CQ. Untreated. Untreated. Untreated. Untreated. Untreated. 76 kDa.

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Mean percentage of polarized mitochondria

(B). (A). GI-LI-N cells. LB24 cells. 30000. Control. Control. ***. 30000. 14 kDa. m. m. D1 (10. M). D1 (10. M). ***. m. m. D6 (5. M). D6 (5. M). ***. 37 kDa. z-VAD-FMK. z-VAD-FMK. 20000. 20000. ***. z-VAD-FMK+D1. z-VAD-FMK+D1. Caspase 3/7 (RFU). Caspase 3/7 (RFU).

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J Freitag et al. Nature 485 , 522-525 (2012) doi:10.1038/nature11051

Dual targeting of GAPDH and PGK occurs in many fungi. J Freitag et al. Nature 485 , 522-525 (2012) doi:10.1038/nature11051.

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3Y1

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MuRF1

Supplementary Figure 2. Day 1. Day 7. Day 30. Orx +E 2. Sham. Orx +v. Sham. Orx +v. Orx +T. Sham. Orx +v. Orx +T. MuRF1. GAPDH. Myostatin. GAPDH.

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HSPG

/. HS. HSPG. LPS. HSPG-hd. iNOS 493 bp. TNF- a 378 bp. GAPDH 983 bp. 1,6. 2. *. *. *. *. 1,6. 1,2. Relative level of (TNF- a /GAPDH). Relative level of (iNOS/GAPDH). 1,2. 0,8. 0,8. 0,4. 0,4. 0. 0. HS. HSPG-hd. HS. HSPG-hd. /. HSPG. LPS. HSPG. LPS. /.

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C 13

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15. C 13. GAPDH.

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GAPDH

TG. WT. p-ERK1/2 / GAPDH (AU). p-AKT / GAPDH (AU). p-AKT. p-ERK1/2.

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GAPDH

A. MDA-MB231. MDA-MB231. MDA-MB231. MCF 7. MCF 7. MCF 7. MsrA. GAPDH. B. MsrA. Sp1. b -actin. b -actin.

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WT S

MI. MI. MI. S. S. S. -. -. -. -. -. -. /. /. /. /. /. /. -. -. -. -. -. -. NOS 1. NOS 1. NOS 1. NOS 1. NOS 1. NOS 1. WT MI. WT MI. WT MI. WT S. WT S. WT S. phox. phox. phox. P67. P67. P67. phox. phox. P47. P47. phox. phox. phox. P22. P22. P22.

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Figure S1

supporting data. A. B. pTyr 99. HFD. control ASO. DEP-1 ASO. kDa. 1.6. 170. 1.4. 130. 1.2. 100. 1.0. 70. 0.8. arbitrary units. pTyr 99. 0.6. 55. 0.4. 0.2. 40. 0. HFD. DEP-1 ASO. control ASO. GAPDH. 35. Figure S1.

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MW Tat 100ng/ml 0h 1h 3h 6h 12h

Flk-1/KDR GAPDH VEGF-189 VEGF-165 VEGF-121. MW Tat 100ng/ml 0h 1h 3h 6h 12h.

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Callus Root Leaf Stem Spike

(a). Mock Salt Cold Heat Drought. (b). Callus Root Leaf Stem Spike. GAPDH. GAPDH. 28S rRNA. 28S rRNA. 18S rRNA. 18S rRNA. (c). Mock IAA ABA ACC GA Zeatin SA BL MeJA. GAPDH. 28S rRNA.

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