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End Repair of double-stranded cDNA

End Repair of double-stranded cDNA

Total RNA isolation. End Repair of double-stranded cDNA. mRNA Isolation using O ligo ( dT ) Magnetic Beads. Adenylation (A-Tailing). Blunt/TA Adaptor Ligation. AAAAAAA. TTTTTTTTT. TTTTTTTTT. AAAAAAA. AAAAAAAAA. AAAAAAAAAAAA. TTTTTTTTTT. TTTTTTTTT. A.

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882 views • 70 slides
Next-Generation Sequencing of Microbial Genomes and Metagenomes

Next-Generation Sequencing of Microbial Genomes and Metagenomes

Next-Generation Sequencing of Microbial Genomes and Metagenomes. Christine King Farncombe Metagenomics Facility Human Microbiome Journal Club July 13, 2012. Overview. Next-generation sequencing Applications Instruments Library prep and sequencing chemistry Sequence quality

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762 views • 41 slides
Course Expectations High-throughput sequencing technology Very large datasets

Course Expectations High-throughput sequencing technology Very large datasets

6/13/19. Course Expectations High-throughput sequencing technology Very large datasets. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research Learn how to use publicly available databases/websites to find specific information about genes

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394 views • 39 slides
Progress towards high-throughput sequencing for clinical HLA genotyping

Progress towards high-throughput sequencing for clinical HLA genotyping

Progress towards high-throughput sequencing for clinical HLA genotyping . Matthew W. Anderson MD, PhD Assistant Professor, Department of Pathology Assistant Director, Stanford Histocompatibility, Immunogenetics, and Disease Profiling Laboratory (HIDPL) Stanford University School of Medicine.

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464 views • 26 slides
Current Sequencing Technologies and Data Generation

Current Sequencing Technologies and Data Generation

Current Sequencing Technologies and Data Generation. Corbin Jones & Piotr Mieczkowski Department of Biology, College of Arts and Sciences, Carolina Center for Genome Sciences Department of Genetics, School of Medicine, University of North Carolina at Chapel Hill. Library prep. Sequencing.

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459 views • 26 slides

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