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CLS 3311 Advanced Clinical Immunohematology

CLS 3311 Advanced Clinical Immunohematology

CLS 3311 Advanced Clinical Immunohematology. Other Blood Groups Kidd, Kell, Duffy and MNSs. Kidd Blood Group System Genetics. Three alleles: Jk a , Jk b , Jk Codominant Inheritance Jk is a silent allele (amorph)

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The work described here was supported by the National Institutes of Health GM 62412

The work described here was supported by the National Institutes of Health GM 62412

On-column Chemical Refolding Natalia Oganesyan and Rosalind Kim Lawrence Berkeley National Laboratory PPCW, March 29-31, 2004. The work described here was supported by the National Institutes of Health GM 62412 . IB in 8 M urea Dilution into a large volume of buffer with detergent

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Towards an Asthma Vaccine: Scalable Process for the Purification of a Latex Allergen Protein

Towards an Asthma Vaccine: Scalable Process for the Purification of a Latex Allergen Protein

Towards an Asthma Vaccine: Scalable Process for the Purification of a Latex Allergen Protein. Presented by : Anushi E. Kulasiri. The Problem. 80% of occupational asthma in the healthcare industry is caused by latex allergies.

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Denaturing Urea Polyacrylamide Gel Electrophoresis

Denaturing Urea Polyacrylamide Gel Electrophoresis

Denaturing Urea Polyacrylamide Gel Electrophoresis. 15% Native PAGE-TBE gel 1M, 2M, 3M Urea gels 36 base pair DNA. 15% Native. Observations: Samples show some excess Cy5 or Cy3. Samples Ligated , Nicked AT, Nicked GC show the same mobility . . 1 M Urea. Observations:

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Isolation of N- linked glycopeptides from plasma

Isolation of N- linked glycopeptides from plasma

SPEG METHOD [1] COOH H 2 N Capturing to Hydrazide beads COOH COOH H 2 N H 2 N Oxidation (NaIO 4 ) COOH H 2 N COOH COOH H 2 N H 2 N Hydrazide captured glycoproteins Oxidized glycans Mouse plasma + [ 14 C]AG/AT Tryptic digestion COOH H 2 N PNGase F cleavage from beads

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