'Ms medium' diaporamas de présentation

Murashige and Skoog (MS medium) Vitamins Minerals Sucrose Kinetin Disinfectant Agar

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MS Medium | TM Media

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Figure 1. Effect of culture cooling on strawberry plant quiescence and endogenous IAA level

Figure 1. Effect of culture cooling on strawberry plant quiescence and endogenous IAA level. IAA (ng g -1 f.w.). Time of cooling (weeks). Figure 2. Hormonal control of bud dormancy in micropropagated strawberry. Dormant auxiliary bud. MS + 0.5 mg l -1 IBA 0.5 mg l -1 BA

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Biochemical analysis of in vitro derived plants of Sterculia urens during acclimatization

Sterculia urens is a commercially important tree with wide applications. Nodal explants were grown on MS medium supplemented with various growth regulators like 6-benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (Kn). These shoots were subsequently rooted in vitro on half strength MS medium containing various concentrations of auxins like Indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA). Plants were acclimatized to ex vitro conditions and established in the field. Role of peroxidase, poly phenol oxidase, total proteins, total carbohydrates and total phenols were studied during acclimatization by estimating their at an interval of 20, 40 and 60 days. Peroxidase and poly phenol oxidase level decreases from 20 to 60 days where as levels of total proteins, carbohydrates and phenols increases.

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In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle

Rheum webbianum commonly known as "rhubarb is a reputed medicinal plant belonging to family Polygonaceae. An attempt has been made to carry out the in vitro regeneration of this important medicinal plant species using hypocotyl segments as explants. Direct organogenesis occurred when the hypocotyl segments from the in vitro germinated seedlings were inoculated on MS medium containing different growth regulators at various concentrations and combinations. Cytokinins like BAP and Kn were found to be effective in shoot regeneration both individually as well as in combination with auxins like IAA, IBA and 2,4 D MS medium supplemented with BAP at a concentration of 2mg l in combination with IAA at a concentration of 3mg l was found to be the best medium for shoot regeneration. For rooting of regenerated shoots half strength MS medium supplemented with 0.5mg l IAA was found to be most effective. Sabreena Rashid | Zahoor A Kaloo | Seema Singh | Iram Bashir "In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: https://www.ijtsrd.com/papers/ijtsrd9558.pdf Paper URL: http://www.ijtsrd.com/biological-science/botany/9558/in-vitro-plant-regeneration-from-hypocotyl-explants-of-rheum-webbianum-royle/sabreena-rashid

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Withaferin-A isolated from the cell cultures of Withania Somnifera

Cell suspension culture of Withania Somnifera was prepared and was analysed for it’s constituents. One Withanolide was isolated from cell suspension culture. The structures of compounds was identified to be Withaferin-A. Based on spectroscopic methods like IR, MASS spectroscopy and H1 NMR spectroscopy all compounds were elucidated.

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Supplementary Figure 2. + - + -. Unteated GA3 treated. + - + -. NAA treated BAP treated.

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Figure 6 Growth of seedlings in vertically oriented square plates

Figure 6 Growth of seedlings in vertically oriented square plates (A) View of the metacrylate platforms used to maintain the verticality of the plates during the experiments performed. (B) View of wild type seedlings grown in square plates on hard agar MS medium

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