'Pcr primers' diaporamas de présentation

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DNA sequencing

DNA sequencing. Part 1: Chemistry, instrumentation and data analysis Part 2: Large-scale operations, comparative sequencing. Part 3: Sequencing analysis, variation analysis. Abbrev 02/03/05. DNA sequencing: Importance. Basic blueprint for life; Aesthetics. Gene and protein. Function

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Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome. Jay Shendure, Gregory J. Porreca, Nikos B. Reppas, Xiaoxia Lin, John P. McCutcheon. Abraham M. Rosenbaum, Michael D. Wang, Kun Zhang, Robi D. Mitra, George M. Church. Science 2005 vol 309, pg 1728-32. Authors.

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Today

Today. House Keeping Course Evaluation Current standing (talk to Coen) Report writing Two *.ppt Minion and fungal genomics Snails and parasites, Genbank submissions. REPORTS in PDF format Due 23.59h, Tuesday December 11th. Grading Key for final report.

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AFLP Amplified Fragment Length Polymorphism

AFLP Amplified Fragment Length Polymorphism. AFLP. - a method based on PCR developed in 1995 by Vos et al. Involves the use of RFLP and PCR techniques Compared with the widely used RFLP, AFLP is faster, less labour intensive and provide more information.

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Initial sample: one or several DNA molecules of interest

Initial sample: one or several DNA molecules of interest. PCR: Polymerase Chain Reaction Ingredients: - many copies of two primers corresponding to the desired start and end location - many copies of nucleotides (C,G,A,T)

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Polymerase Chain Reaction

Polymerase Chain Reaction. What is PCR History of PCR How PCR works Optimizing PCR Fidelity, errors & cloning PCR primer design Application of PCR. Adaptor Ligation. Two different adaptors (short double stranded DNA sequences with sticky end) are ligated to the digested fragments.

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Undifferentiated

Differentiated (4 d). Undifferentiated. Supplemental Figure S1. Supplemental Figure S2. TRE. GC box. Supplemental Figure S3. A. a. b. c. d. e. 8 d. N5. N4. N3. N2. N1. 0 d. ApaI. XhoI. PstI. SmaI. ApaI. f. g. h. i. PstI. ApaLI. PstI. SpeI. B. a/b. Xho1. a/c. N5.

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Identifying exons for PCR primer design in Plasmodium falciparum

gene. exon. exon. exon. intron. intron. transcription. mRNA prior to processing. mRNA processing. Processed mRNA. translation. protein. Identifying exons for PCR primer design in Plasmodium falciparum.

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Supplementary Material 2. PCR primers used in this study.

Supplementary Material 2. PCR primers used in this study.

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Supplemental Table 1

Supplemental Table 1.

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31 87 pSAT4.hspP. EGFP

Clone number in the collection. Clone number in the collection. Vector name. Insert template. 2 298 pSAT6.35SP.MCS.35ST. 31 87 pSAT4.hspP. EGFP. BamHI - EGFP -F SpeI - EGFP -R. CGATGAGGATCCGTGAGCAAGGGCGAGGAGC. GATACAACTAGTCTTGTACAGCTCGTCCATG. Restriction enzymes. PCR primers. BamHI

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Supplemental Table 1

Supplemental Table 1.

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