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Enzymes required for recombination

Enzymes required for recombination

Enzymes required for recombination. Overview Generation of single strands Invasion of single strands Branch migration Resolution. Identification of enzymes required for recombination. Screen bacteria and yeast for mutants with decreased recombination frequency

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385 views • 23 slides
Genomic Rescue: Restarting failed replication forks

Genomic Rescue: Restarting failed replication forks

Genomic Rescue: Restarting failed replication forks. Andrew Pierce Microbiology, Immunology and Molecular Genetics University of Kentucky. MI/BCH/BIO 615. RecA. Binds single-stranded DNA and double-stranded DNA Searches for regions of homology Exchanges homologous strands. +. RecA.

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309 views • 18 slides
Homologous Recombination

Homologous Recombination

Homologous Recombination. MCB 7200 Group 1 Ashley Patton Yanyang Cuo Samantha Shaw. Homologous Recombination. Biological Relevance Repairs double-stranded breaks Chromosome segregation during meiosis I Genetic diversity in gametes Mediated by two important enzymes Rad51 and Dmc1

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718 views • 14 slides
The Role of RecA in DNA Replication

The Role of RecA in DNA Replication

The Role of RecA in DNA Replication. Alastair Plant Salah Awad. Discovery of RecA. Clark (1967) screened E. coli colonies for mutants with impaired recombination. These mutants were UV-sensitive. Homologous genes exist in prokaryotes and eukaryotes (e.g.Rad51).

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263 views • 14 slides
Homologous Recombination

Homologous Recombination

Homologous Recombination. MCB 7200 Group 1 Ashley Patton Yanyang Cuo Samantha Shaw. Homologous Recombination. Biological Relevance Repairs double-stranded breaks Chromosome segregation during meiosis I Genetic diversity in gametes Mediated by two important enzymes Rad51 and Dmc1

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238 views • 14 slides
Homologous Recombination

Homologous Recombination

Homologous Recombination. MCB 7200 Group 1 Ashley Patton Yanyang Cuo Samantha Shaw. Homologous Recombination. Biological Relevance Repairs double-stranded breaks Chromosome segregation during meiosis I Genetic diversity in gametes Mediated by two important enzymes Rad51 and Dmc1

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267 views • 14 slides
recA

recA

recA. an evolutionary investigation Andrew Lloyd Bacterial Gene Day 2006. Why recA. Lloyd, A.T. and Sharp, P.M. (1993) Evolution of the recA gene and the molecular phylogeny of bacteria. J. Mol. Evol. 37:399-407. It was the most widely sequenced protein 23 genes available in 1993

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235 views • 6 slides
KE Duderstadt et al . Nature 000 , 1 - 5 (2011) doi:10.1038/nature10455

KE Duderstadt et al . Nature 000 , 1 - 5 (2011) doi:10.1038/nature10455

DnaA engages ssDNA in a manner similar to RecA. KE Duderstadt et al . Nature 000 , 1 - 5 (2011) doi:10.1038/nature10455.

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87 views • 1 slides

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