'Restriction map' diaporamas de présentation

Chapter 2: From genes to Genomes

Chapter 2: From genes to Genomes. 2.1 Introduction. Mapping: Genetic (or linkage) map : recombination frequencies Restriction map : length of DNA Ultimate map: sequence of the DNA overlapping fragments of DNA:. 2.2 Genes can be mapped by restriction cleavage. 5kb. Figure 2.1

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DNA Mapping and Brute Force Algorithms

DNA Mapping and Brute Force Algorithms. Outline. Restriction Enzymes Gel Electrophoresis Partial Digest Problem Brute Force Algorithm for Partial Digest Problem Branch and Bound Algorithm for Partial Digest Problem. Molecular Scissors. Molecular Cell Biology, 4 th edition.

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Announcements

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Computational Genomics Course Lecture 12 fall 2002/03 School of Computer Science Tel-Aviv University Instructor: Benny

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Solving Double Digest Problem by Genetic Algorithm

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Restriction Mapping

Restriction Mapping. Central Dogma. Human Migration. Gel Electrophoresis. Restriction Enzymes : Cut at specific and unique sequences called Restriction Sites. Restriction Site Mapping Like Solving a puzzle. Boreal Toads Bufo boreas boreas. Learning Goals.

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Making a Restriction Map

Making a Restriction Map . AP BIO. Step One. Look at the gel and determine the size fragments that are present in each lane. . BamHI 7 kb, 3 kb EcoRI 7 kb, 3 kb EcoRI + BamHI 3 kb, 2 kb. Step Two-Three. Draw a circle

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Labs 10 to 12

Labs 10 to 12. Southern Blotting & Physical Mapping. 3.4 What Can You Do with a Cloned Gene? Applications of Recombinant DNA Technology. Gel Electrophoresis and Gene Mapping Map of the gene Determine which restriction enzymes cut the gene and pinpoint the exact location of the sites

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