'T7 rnap' diaporamas de présentation

Living Hardware to Solve the Hamiltonian Path Problem

Living Hardware to Solve the Hamiltonian Path Problem. Faculty : Drs. Malcolm Campbell, Laurie Heyer, Karmella Haynes. Faculty : Drs. Todd Eckdahl, Jeff Poet.

693 views • 57 slides

Lingchong You

BME 265-05. March 31, 2005. Modeling T7 life cycle. Lingchong You. Individual appointments (1hr/group) next week. Project report due today!. Monday: 1pm-6pm Tuesday: 9:30am-11:30am & 1:30-5:30pm. Bacteriophages: landmarks in molecular biology. 1939 one-step growth of viruses

667 views • 50 slides

Expression systems

Lecture 7. Expression systems. Designing vectors and making protein. Few slides by David Tscharke @ RSB. Lecture overview. Ingredients of an overexpression system (cloning) Growing E. coli Induction of protein expression. The ultimate overexpression system. High copy-number plasmid

519 views • 31 slides

Michaelis-Menten Kinetics

Michaelis-Menten Kinetics. K m is the substrate concentration at which the rate of the reaction is half the maximum rate (V max ). Transcription. 2-10 nM. 9-12 nt synthesis. 1x10 8 M -1 s -1. 30s -1. 150s -1. bent. F obs = F o (1- e -kt )+F b. Expected results. fluorescence. time.

2.94k views • 27 slides

General Considerations Expression systems Protein Fusion / Purification

93 views • 8 slides