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Mechanical Strain and Growth Factors To Stimulate SMC Growth

Mechanical Strain and Growth Factors To Stimulate SMC Growth. Prepared by Seth Hills. Introduction. Atherosclerosis Costs-Treatments Artery Composition. Natural Materials Growth Factors Cyclic Strain Use them together to enhance SMC growth. Biocompatible tissue engineered vascular graft.

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Mechanical Strain and Growth Factors To Stimulate SMC Growth

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  1. Mechanical Strain and Growth Factors To Stimulate SMC Growth Prepared by Seth Hills

  2. Introduction • Atherosclerosis • Costs-Treatments • Artery Composition

  3. Natural Materials Growth Factors Cyclic Strain Use them together to enhance SMC growth Biocompatible tissue engineered vascular graft Our Strategy: Our Goal:

  4. Why are we studying this? • They haven’t been studied together. • Growth factors (GFs) stimulate SMC growth. • Similar to in vivo • Potential additive effects • Understand mechanism of atherosclerosis

  5. Methods: • Tissue Culturing Substrates • Cell Number vs. Cell DNA • GF Concentrations vs. Cell Proliferation

  6. Growth Factors • TGFβ(Transforming Growth Factor Beta) • PDGF (AA, AB, BB) (Platelet Derived Growth Factor) • FGF (Fibroblast Growth Factor) • VEGF (Vascular Endothelial Growth Factor) • IL-2(Interleukin 2)

  7. Results:

  8. Future Work • Mold design for vascular graft • Factorial Designs • Design pulsatile flow system

  9. Vascular Graft Molds

  10. Factorial Designs • Mechanical Testing • Study the effect of cyclic strain, compared to static controls, on SMCs with and without GFs. • Vascular Graft Composition: • Optimize for good mechanical properties • Cell encapsulation

  11. Cyclic Strain • Vacuum provides stretch • Induces biochemical changes in response to strain

  12. Mechanical Testing

  13. Vascular Graft Composition Full Factorial Design Parameters Half Factorial *for splitting into a half factorial

  14. Flow System (Jeng-Jiann Chiu)

  15. Vascular Graft (Cummings)

  16. Methods: Substrate Study • Seed cells at 96,000 cells/well in BioFlex™ 6 well plates of different substrate along with a control. • When cells are confluent in one of the substrates Lyse the cells and perform DNA assay.

  17. Methods: Cell Number vs. Cell DNA • Day 1: Seed cells at different concentrations in 24 well plate • Day 2: Lyse cells and perform DNA assay

  18. Methods: GF Concentration Study • Day 1: Seed cells in 24 well plates at 20,000 cells/well. • Day 2: Feed cells with quiescent media. • Day 3: Begin feeding cells with different GF concentrations. • Feed every other day • Lyse cells when they are confluent in one of the concentrations and perform DNA assay.

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