Graph A Graph B Graph C A different shape not shown here

1. Protein A is allosterically regulated by molecule X. You mix samples of Protein A with increasing concentrations of molecule X. Which graph best represents Protein A activity? Graph A Graph B Graph C A different shape not shown here

2. Protein A is negatively regulated by allosteric binding of molecule X. You mix samples of Protein A with increasing concentrations of molecule X. Draw what the graph of Protein A activity would look like, holding the concentration of Protein A constant.

3. A protein is made of 100 amino acids. The following is true: • A. the protein has 98 peptide bonds • B. the protein has 98 R groups • C. the protein has 98 domains • D. none of the above • You are analyzing two versions of an enzyme that breaks down proteins, • one from crickets and one from horse liver. You put in equal concentrations of • each enzyme in two test tubes (at room temperature) with the same protein substrate, • and find that the horse enzyme breaks down the protein more slowly than the cricket enzyme. • What could be the reason(s) for this? • A. the horse enzyme has a different amino acid sequence than the cricket enzyme • B. the horse enzyme works better at a higher temperature than the cricket enzyme • C. the cricket enzyme has a different tertiary structure from the horse enzyme • D. all of the above are possible • If you replace a basic amino acid in a protein with an acidic one, the following might • happen to the protein: • A. nothing • B. the protein might fold differently • C. the protein might behave differently • D. all of the above