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PCR Polymerase Chain Reaction

PCR Polymerase Chain Reaction. a tool to generate a large amount of a gene of interest. DNA-sequence in a plasmid. DNA. . . Restriction of plasmid releases the DNA sequence of interest. Plasmid. P olymerase C hain R eaction

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PCR Polymerase Chain Reaction

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  1. PCR Polymerase Chain Reaction a tool to generate a large amount of a gene of interest

  2. DNA-sequence in a plasmid DNA   Restriction of plasmid releases the DNA sequence of interest Plasmid Polymerase Chain Reaction synthesizes only the gene of interest by using Forward and Reverse primers (specific small pieces of DNA) with the help of the enzyme Polymerase. All cycles follow a strict order of timed temperatures. First cDNA strand Second cDNA strand Forward primer Reverse primer Gene of Interest

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