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Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS

Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS. Pre-lab activity. DNA isolation. Transform. Express. Clone. Purify. Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS. Title goes at the top of the first page

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Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS

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  1. Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS Pre-lab activity DNA isolation Transform Express Clone Purify

  2. Lab 4: VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS • Title goes at the top of the first page • Each section must be clearly underlined • Skip lines between each section • Use blue or black PEN! • Follow these directions. You must be ready for lab (including pre-lab questions) at the beginning of the period. • 1. Title: copy this title...” VERIFICATION OF RESTRICTION AND LIGATION USING GEL ELECTROPHORESIS ” • 2. Background: write a definition for the following words: supercoiled plasmid, nicked-circle, multimer • 3. Pre-lab questions: Copy and answer the BEFORE THE LAB #s 1-3 questions on page B-41. • 4. Objective: Read the background information and determine the objective of the lab. • 5. Hypothesis: Create your hypothesis. • 6. Materials: copy the materials in a bulletedlist • 7. Procedure: Create a flowchart of the procedure. • 8. Data : Take a picture of your results. Print and label. • 9. Data Analysis: Copy & answer the STOP & THINK Qs on page B-43 & B-45.. • 10. Conclusion: Copy & answer the 9 questions on page B-46.

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