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FISH in the CRC: A Report on Pilot Studies

FISH in the CRC: A Report on Pilot Studies. Donna Neuberg May 6, 2006. Participating Cytogeneticists. Organized by Gordie Dewald (Mayo) Marie Dell’Aquila (UCSD) Paola Dal Cin (BWH) Lynne Abruzzo (MDACC) Nyla Heerema (OSU) Prasad Kodur (LIJ) Connie Griffin (JHOC). To Date:.

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FISH in the CRC: A Report on Pilot Studies

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  1. FISH in the CRC:A Report on Pilot Studies Donna Neuberg May 6, 2006

  2. Participating Cytogeneticists • Organized by Gordie Dewald (Mayo) • Marie Dell’Aquila (UCSD) • Paola Dal Cin (BWH) • Lynne Abruzzo (MDACC) • Nyla Heerema (OSU) • Prasad Kodur (LIJ) • Connie Griffin (JHOC)

  3. To Date: • Survey of how FISH is currently done at the institution, and what resources are in place • Pilot 1: 10 cases circulated for FISH, however it is currently done • Pilot 2: 2 cases circulated to try ND-FISH

  4. Survey • Variability in • Equipment • Processing • Scoring strategy • Everyone seeks to score 200 interphase nuclei per hybridization • Cutoffs for normal differ across institutions

  5. Cutoffs (%)

  6. Pilot 1 • FISH as standardly done in that lab • 10 cases • 4 slides for each case • 2 pre-etched 15 mm circles so 2 FISH assays per slide • One control sample included (normal donor) • Efficacy and accuracy • 5 V-FISH (Vysis), 1 ND-FISH

  7. -17/17p-

  8. -11/11q-

  9. t12

  10. 13q-x1

  11. Observations—Pilot 1 • All sites were quite good • Missed anomalies (won’t find if don’t look) • Pt 8 had 65% 6q- • ND-FISH distinguished -11 and 11q- in pt 2, and +MDM2 in pt 3 • Scoring inconsistencies • Aqua • Number of cells scored

  12. Summary—Pilot 1 • Excellent results across the board • Identify issues for further discussion and piloting

  13. Pilot 2 • Gain experience with ND-FISH (Mayo std) • Can distinguish monosomy from deletion on ch 17 and 11 • Includes • 6q- • +MDM2 • 13q-x1, 13q-x2, +13q34 • IgH abn

  14. Summary • First experience with ND-FISH went exceptionally well • Need more practice on monosomy vs deletion • Need more accuracy to address prognostic significance • More variability if percentage mid-range • Need to address if % abn will be important • Development of metaphase FISH in CLL

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